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<p>Chronic inflammation, decreased tissue regeneration, and reduced antimicrobial efficacy make bacterial biofilm-associated wound infections a major therapeutic concern. Infected wounds can be treated with a biopolymeric nanocomposite hydrogel that incorporates AgLNPs. A probe-sonication-assisted green technique using <i>Schleichera oleosa</i> fruit extract produced AgLNPs, which were encapsulated in lipids. The nanoparticles were then added to a cross-linked okra–fenugreek polysaccharide hydrogel matrix for biocompatibility and sustained activity. The produced AgLNPs had a hydrodynamic diameter of 141&#xa0;nm, a polydispersity index of 0.248, and a zeta potential of −&#xa0;29.6&#xa0;mV, indicating good colloidal stability. Comprehensive morphological and structural investigations using FESEM, HRTEM, FTIR, XRD, and DLS confirmed lipid coating and nanoparticle uniformity in the hydrogel network. This nanocomposite hydrogel showed pH-responsive swelling and good hemocompatibility, with hemolysis &lt; 1%. Broad-spectrum antibacterial activity against <i>Staphylococcus aureus</i>, <i>Bacillus simplex</i>, and <i>Escherichia coli</i> and biofilm inhibition was found against biofilm-forming bacteria. Anti-inflammatory assessment showed dose-dependent protein denaturation inhibition and increased THP-1 cell interleukin-10 production. Cytocompatibility testing by L929 fibroblast cells showed high cell viability and proliferation. In mice infected with biofilms, wound contraction was hastened accompanying complete wound closure and fibroblast density, and collagen deposition was higher than in untreated controls and a conventional treatment group.</p> Graphical abstract <p></p>

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Lipid-coated silver nanoparticles embedded in a biopolymer okra–fenugreek hydrogel for biofilm infected wound healing

  • 期刊论文

  • 发表日期：

    2026年7月9日

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  • Kajal Kumari,
  • Muthu Kumar Sampath,
  • Hare Ram Singh,
  • Trishna Bal,
  • Itishree Jogamaya Das

摘要

Chronic inflammation, decreased tissue regeneration, and reduced antimicrobial efficacy make bacterial biofilm-associated wound infections a major therapeutic concern. Infected wounds can be treated with a biopolymeric nanocomposite hydrogel that incorporates AgLNPs. A probe-sonication-assisted green technique using Schleichera oleosa fruit extract produced AgLNPs, which were encapsulated in lipids. The nanoparticles were then added to a cross-linked okra–fenugreek polysaccharide hydrogel matrix for biocompatibility and sustained activity. The produced AgLNPs had a hydrodynamic diameter of 141 nm, a polydispersity index of 0.248, and a zeta potential of − 29.6 mV, indicating good colloidal stability. Comprehensive morphological and structural investigations using FESEM, HRTEM, FTIR, XRD, and DLS confirmed lipid coating and nanoparticle uniformity in the hydrogel network. This nanocomposite hydrogel showed pH-responsive swelling and good hemocompatibility, with hemolysis < 1%. Broad-spectrum antibacterial activity against Staphylococcus aureus, Bacillus simplex, and Escherichia coli and biofilm inhibition was found against biofilm-forming bacteria. Anti-inflammatory assessment showed dose-dependent protein denaturation inhibition and increased THP-1 cell interleukin-10 production. Cytocompatibility testing by L929 fibroblast cells showed high cell viability and proliferation. In mice infected with biofilms, wound contraction was hastened accompanying complete wound closure and fibroblast density, and collagen deposition was higher than in untreated controls and a conventional treatment group.

Graphical abstract