<p>The aim of this study is to assess potential alterations in magnetic strength of NdFeB magnets after sterilization. For the first time, we report an investigation of the impact of low-temperature sterilization using a hydrogen peroxide-based protocol (STERRAD) on different grades and sizes of NdFeB magnets representative of those available on the market (38EH, 45UH, and 50SH). Conventional high-temperature steam autoclave sterilization was also examined. These protocols were studied to determine both potential thermal demagnetization and chemical impact of free radicals generated during H₂O₂ sterilization on NdFeB magnets. Magnetization strength was measured before and after each sterilization protocol using a gaussmeter introduced into a custom magnet positioning device, providing a quantitative assessment of sterilization-induced changes. No clinically relevant change in magnetization strength was observed following either autoclave or STERRAD sterilization, with magnets sterilized individually showing variations similar to those sterilized in groups, indicating consistent performance across different sterilization conditions. Sterilization of NdFeB magnets by autoclave or STERRAD procedures does not result in significant alteration of magnetic strength. Assessment of additional sterilization techniques is required to confirm these findings across broader conditions.</p> Graphical abstract <p></p>