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<p>The papain-like protease of SARS-CoV-2 (PLpro2) is integral to viral polyprotein cleavage and the modulation of host immune responses, positioning it as a critical target for antiviral drug development. Here, we elucidate the molecular mechanisms governing the noncovalent inhibition of PLpro2 through a comprehensive computational approach, including molecular docking, extensive molecular dynamics (MD) simulations, binding free energy calculations (MM/GBSA and SIE), principal component and free energy landscape (PCA/FEL) analyses, and protein–ligand interaction fingerprinting (ProLIF). We assessed a structurally diverse set of noncovalent inhibitors for their capacity to induce conformational rearrangements and stabilize key structural motifs of PLpro2, with particular emphasis on the BL2 loop. Notably, XR3 and A19 exhibited superior experimental and predicted binding affinities, which can be attributed to favorable contacts with essential residues Tyr268 and Gln269, the attenuation of loop dynamics, and the stabilization of energetically favorable conformational states. By contrast, less potent inhibitors were associated with increased conformational heterogeneity, fragmented free energy landscapes, and diminished interactions with critical loop residues. Therefore, our integrative analysis delineates the structural and energetic determinants underpinning noncovalent PLpro2 inhibition, underscoring the central roles of loop immobilization and π-stacking interactions in the rational design of next-generation PLpro2 inhibitors.</p>

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Mechanistic insights into the noncovalent inhibition of SARS-CoV-2 PLpro: a multiscale computational study

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月5日

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  • Flávio Vinícius da Silva Ribeiro,
  • Renan Patrick da Penha Valente,
  • Hendrik G. Kruger,
  • Jéssica de Oliveira Araújo,
  • José Rogério A. Silva

摘要

The papain-like protease of SARS-CoV-2 (PLpro2) is integral to viral polyprotein cleavage and the modulation of host immune responses, positioning it as a critical target for antiviral drug development. Here, we elucidate the molecular mechanisms governing the noncovalent inhibition of PLpro2 through a comprehensive computational approach, including molecular docking, extensive molecular dynamics (MD) simulations, binding free energy calculations (MM/GBSA and SIE), principal component and free energy landscape (PCA/FEL) analyses, and protein–ligand interaction fingerprinting (ProLIF). We assessed a structurally diverse set of noncovalent inhibitors for their capacity to induce conformational rearrangements and stabilize key structural motifs of PLpro2, with particular emphasis on the BL2 loop. Notably, XR3 and A19 exhibited superior experimental and predicted binding affinities, which can be attributed to favorable contacts with essential residues Tyr268 and Gln269, the attenuation of loop dynamics, and the stabilization of energetically favorable conformational states. By contrast, less potent inhibitors were associated with increased conformational heterogeneity, fragmented free energy landscapes, and diminished interactions with critical loop residues. Therefore, our integrative analysis delineates the structural and energetic determinants underpinning noncovalent PLpro2 inhibition, underscoring the central roles of loop immobilization and π-stacking interactions in the rational design of next-generation PLpro2 inhibitors.