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Purpose <p>To determine the impact of low versus atmospheric oxygen tension during only the pre-maturation phase of biphasic capacitation in vitro maturation.</p> Methods <p>The study involved sibling oocytes (532 cumulus-oocyte complexes [COCs] from 20 participants [mean age 29.5 ± 2.5&#xa0;years]) with polycystic ovary syndrome undergoing CAPA-IVM without gonadotrophins. After oocyte pick-up (OPU), COCs were randomized to undergo pre-maturation under low or atmospheric oxygen, then IVM culture at 20% oxygen. The primary outcome was the oocyte maturation rate. Secondary outcomes included rates of fertilization, blastulation, and good-quality blastocysts. Cumulus cell gene expression was also determined, and glucose and lactate concentrations in spent culture media were analyzed.</p> Results <p>Oocyte maturation rates per COC (59.8% vs. 56.1%; <i>p</i> = 0.47) and per patient (61% vs. 55%; <i>p</i> = 0.34) were comparable between the atmospheric vs. low oxygen groups. Fertilization rates were significantly higher in the atmospheric versus low oxygen group; all other embryology outcomes were similar between groups. Significantly different cumulus cell gene expression was seen for <i>LDHA</i> (glucose metabolism; lower in the low oxygen group), <i>CPT1A</i> (lipid shuttle; higher in the low oxygen group), and <i>NDRG1</i> (hypoxia response; lower in the low oxygen group). Glucose consumption and lactate production were comparable between groups during pre-maturation, but lower in the low oxygen group during IVM.</p> Conclusions <p>Overall, although low oxygen tension during CAPA-IVM pre-maturation reduces the number of fertilized oocytes, it does not compromise fertilized oocyte quality or embryological outcomes.</p>

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Use of low versus high oxygen tension during pre-maturation only in capacitation in vitro maturation (CAPA-IVM): impact on oocyte maturation and metabolism

  • 期刊论文

  • 发表日期：

    2026年4月30日

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  • Kha T. Huynh,
  • Dung T. P. Nguyen,
  • Anh H. Le,
  • Nhu T. M. Khong,
  • Toan D. Pham,
  • Diem T. N. Nguyen,
  • Minh H. N. Nguyen,
  • Ho L. Le,
  • Tien K. Le,
  • Bao G. Huynh,
  • Nhung H. Truong,
  • Ellen Anckaert,
  • Johan E. J. Smitz,
  • Robert B. Gilchrist,
  • Tuong M. Ho,
  • Nazli Akin,
  • Lan N. Vuong

摘要

Purpose

To determine the impact of low versus atmospheric oxygen tension during only the pre-maturation phase of biphasic capacitation in vitro maturation.

Methods

The study involved sibling oocytes (532 cumulus-oocyte complexes [COCs] from 20 participants [mean age 29.5 ± 2.5 years]) with polycystic ovary syndrome undergoing CAPA-IVM without gonadotrophins. After oocyte pick-up (OPU), COCs were randomized to undergo pre-maturation under low or atmospheric oxygen, then IVM culture at 20% oxygen. The primary outcome was the oocyte maturation rate. Secondary outcomes included rates of fertilization, blastulation, and good-quality blastocysts. Cumulus cell gene expression was also determined, and glucose and lactate concentrations in spent culture media were analyzed.

Results

Oocyte maturation rates per COC (59.8% vs. 56.1%; p = 0.47) and per patient (61% vs. 55%; p = 0.34) were comparable between the atmospheric vs. low oxygen groups. Fertilization rates were significantly higher in the atmospheric versus low oxygen group; all other embryology outcomes were similar between groups. Significantly different cumulus cell gene expression was seen for LDHA (glucose metabolism; lower in the low oxygen group), CPT1A (lipid shuttle; higher in the low oxygen group), and NDRG1 (hypoxia response; lower in the low oxygen group). Glucose consumption and lactate production were comparable between groups during pre-maturation, but lower in the low oxygen group during IVM.

Conclusions

Overall, although low oxygen tension during CAPA-IVM pre-maturation reduces the number of fertilized oocytes, it does not compromise fertilized oocyte quality or embryological outcomes.