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Purpose <p>The objective of this study was to compare the effects of two warming protocols (three step vs. one-step) on re-expansion and post-warming survival of human blastocysts previously vitrified using the standard vitrification protocol.</p> Methods <p>One hundred vitrified blastocysts were included in the study; 50 blastocysts were warmed using the standard warming protocol, and 50 were warmed using the diluted one-step warming protocol (DS 37&#xa0;°C, 3&#xa0;min; WS RT 5&#xa0;min; culture dish until embryo transfer).</p> Results <p>There was no significant difference in survival 2&#xa0;h post-warming between the standard warming protocol and the diluted one-step warming protocol (98% vs 94%; <i>p</i> = 0.3), nor was there any difference in the number of embryos that were completely re-expanded, hatching or hatched when evaluated 2&#xa0;h post-warming (84% vs 80%; <i>p</i> = 0.6). Those results indicate that vitrified blastocysts were able to survive equally between the two investigated protocols.</p> Conclusion <p>The diluted one-step warming of vitrified blastocysts in 0.5&#xa0;M sucrose showed no negative effects on embryo re-expansion and post-warming embryo survival when compared with the standard warming method. Therefore, the elimination of the first step of the standard warming procedure (TS, 37&#xa0;°C, 1&#xa0;min), the most difficult at a practical level, not only allows the reduction of the execution time, but above all, reduces the learning curve while maintaining effectiveness.</p>

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A diluted one-step warming protocol: survival of vitrified blastocyst

  • 期刊论文

  • 发表日期：

    2025年3月5日

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  • Emanuele Licata,
  • Matthew VerMilyea,
  • Brooke Ducote,
  • Tonya Ferguson,
  • Claudia Bianco,
  • Mariagrazia Gallo,
  • Gemma Paciotti,
  • Renata Passerini,
  • Caterina Meneghini,
  • Cristina Fabiani,
  • Francesco Galanti,
  • Alessandro Dal Lago,
  • Rocco Rago

摘要

Purpose

The objective of this study was to compare the effects of two warming protocols (three step vs. one-step) on re-expansion and post-warming survival of human blastocysts previously vitrified using the standard vitrification protocol.

Methods

One hundred vitrified blastocysts were included in the study; 50 blastocysts were warmed using the standard warming protocol, and 50 were warmed using the diluted one-step warming protocol (DS 37 °C, 3 min; WS RT 5 min; culture dish until embryo transfer).

Results

There was no significant difference in survival 2 h post-warming between the standard warming protocol and the diluted one-step warming protocol (98% vs 94%; p = 0.3), nor was there any difference in the number of embryos that were completely re-expanded, hatching or hatched when evaluated 2 h post-warming (84% vs 80%; p = 0.6). Those results indicate that vitrified blastocysts were able to survive equally between the two investigated protocols.

Conclusion

The diluted one-step warming of vitrified blastocysts in 0.5 M sucrose showed no negative effects on embryo re-expansion and post-warming embryo survival when compared with the standard warming method. Therefore, the elimination of the first step of the standard warming procedure (TS, 37 °C, 1 min), the most difficult at a practical level, not only allows the reduction of the execution time, but above all, reduces the learning curve while maintaining effectiveness.