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<p>A simple, effective, and accurate visible-spectroscopic technique has been established to evaluate vitamin H (VIT-H) in medicinal samples as well as in its pure form. The method depends on the inhibitory influence of VIT-H on the Hg<sup>2+</sup>-facilitated ligand substitution (LS) reaction between phenylhydrazine (PHZ) and [Fe(CN)6]4–. The strategy involves substituting CN<sup>–</sup> with PHZ in [Fe(CN)<sub>6</sub>]<sup>4–</sup>, resulting in the formation of a [Fe(CN)<sub>5</sub> PHZ]<sup>3–</sup> complex. The VIT-H can be quantified between 0.017 and 1.710 μg/mL by monitoring the absorbance of the complex at 488 nm during the process. The established method enables the detection of VIT-H at concentrations as low as 0.163 μg/mL. Recuperation experiments confirmed the precision and accuracy of the method for VIT-H quantification. The proposed methodology has been successfully employed for the assessment of VIT-H in pure samples and diverse pharmaceuticals, showcasing remarkable accuracy and precision. The results corresponded with the prescribed analytical protocol. The suggested methodology remains unaffected by the excipients typically employed in medications.</p>

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Kinetic Spectrophotometric Quantification of Vitamin H in Pristine and Drug Formulations in Micellar Medium

  • 期刊论文

  • 发表日期：

    2026年2月28日

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  • Abhishek Srivastava,
  • Ajaya Bhattarai,
  • Pradeep Kumar Pandey,
  • Neetu Srivastava

摘要

A simple, effective, and accurate visible-spectroscopic technique has been established to evaluate vitamin H (VIT-H) in medicinal samples as well as in its pure form. The method depends on the inhibitory influence of VIT-H on the Hg2+-facilitated ligand substitution (LS) reaction between phenylhydrazine (PHZ) and [Fe(CN)6]4–. The strategy involves substituting CN– with PHZ in [Fe(CN)6]4–, resulting in the formation of a [Fe(CN)5 PHZ]3– complex. The VIT-H can be quantified between 0.017 and 1.710 μg/mL by monitoring the absorbance of the complex at 488 nm during the process. The established method enables the detection of VIT-H at concentrations as low as 0.163 μg/mL. Recuperation experiments confirmed the precision and accuracy of the method for VIT-H quantification. The proposed methodology has been successfully employed for the assessment of VIT-H in pure samples and diverse pharmaceuticals, showcasing remarkable accuracy and precision. The results corresponded with the prescribed analytical protocol. The suggested methodology remains unaffected by the excipients typically employed in medications.