<p>A laser spectrofluorimeter for spectral-kinetic luminescence analysis has been developed. The spectrofluorimeter allowed steady-state fluorescence spectra and fluorescence decay kinetics to be recorded using a time-correlated single photon counting method with a time measurement range of 0.2–10,000 ns. Laser diodes (wavelength 400.7, 451.6, 508.2, 657.9, and 759.3 nm) with the ability to adjust the frequency to 0–20 MHz and the pulse length at half-height to 70–200 ps or with the light power increased by 50–100 times with a length of 1.5–3.0 ns and LEDs (267.5, 305.0, and 368.1 nm) with a pulse length at half-height from 1.6 ns were used as fluorescence excitation sources. The optical scheme of the spectrofluorimeter was based on a monochromator-spectrograph with two output ports on which a CMOS detector and a photomultiplier were installed. The spectrofluorimeter in the monochromator mode allowed luminescence in the range 200–900 nm to be recorded; in the polychromator mode, 200–1000 nm. All main units were controlled by a single software program that included the developed software module FluoTau for analyzing the fluorescence decay kinetics. This module allowed the recorded fluorescence decay kinetics to be approximated with a total of up to 5 exponents and had wide capabilities for preliminary processing and setting up the data approximation model. It was shown based on experimental results for eight samples that the spectrofluorimeter allowed luminescence decay times in the range 0.2–10,000 ns to be measured with a time resolution of &lt;0.1 ns.</p>