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<p>The attached cultivation method has recently gained prominence due to the advantages in superior biomass productivity, water conservation and improved harvesting efficiency. Among various factors that influence microalgal growth, temperature is a critical determinant. This study examined the heat responses of attached-cultivated <i>Arthrospira platensis</i>, identifying 25-30°C as the optimal growth range. Extreme temperatures severely impaired biomass accumulation, chlorophyll <i>a</i> synthesis, and photosynthetic efficiency. Chlorophyll fluorescence analysis revealed significant declines in the maximum photochemical efficiency F<sub>v</sub>/F<sub>m</sub> at 45°C, alongside disrupted electron transport and photoprotective capacity. Rubisco activity exhibited transient activation at 40°C and declining at 45°C, indicating thermal destabilization of carbon fixation. Transcriptomic analysis identified a hierarchical stress response, with severe heat (45°C, compared with 30℃) triggering 2449 differentially expressed genes, including downregulation of photosynthetic components (Rubisco, PSII proteins) and upregulation of stress-adaptive pathways. These findings demonstrate that <i>A. platensis</i> exhibits partial thermotolerance in attached cultivation but suffers severe photosynthetic and metabolic damage above 40°C, establishing a mechanistic framework for optimizing <i>A. platensis</i> productivity under heat stress and underscore the need for thermotolerance strategies in climate change scenarios.</p>

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Physiological and transcriptomic responses of attached-cultivated Arthrospira platensis to heat stress

  • 期刊论文

  • 发表日期：

    2025年11月10日

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  • Wen Liu,
  • Lu Li,
  • Youzhi Yu,
  • Zhihui Li,
  • Lin Chen,
  • Tianzhong Liu

摘要

The attached cultivation method has recently gained prominence due to the advantages in superior biomass productivity, water conservation and improved harvesting efficiency. Among various factors that influence microalgal growth, temperature is a critical determinant. This study examined the heat responses of attached-cultivated Arthrospira platensis, identifying 25-30°C as the optimal growth range. Extreme temperatures severely impaired biomass accumulation, chlorophyll a synthesis, and photosynthetic efficiency. Chlorophyll fluorescence analysis revealed significant declines in the maximum photochemical efficiency Fv/Fm at 45°C, alongside disrupted electron transport and photoprotective capacity. Rubisco activity exhibited transient activation at 40°C and declining at 45°C, indicating thermal destabilization of carbon fixation. Transcriptomic analysis identified a hierarchical stress response, with severe heat (45°C, compared with 30℃) triggering 2449 differentially expressed genes, including downregulation of photosynthetic components (Rubisco, PSII proteins) and upregulation of stress-adaptive pathways. These findings demonstrate that A. platensis exhibits partial thermotolerance in attached cultivation but suffers severe photosynthetic and metabolic damage above 40°C, establishing a mechanistic framework for optimizing A. platensis productivity under heat stress and underscore the need for thermotolerance strategies in climate change scenarios.