Antimicrobial activity of the AS-48 peptide produced in Chlamydomonas reinhardtii against Listeria monocytogenes
摘要
Increasing microbial antibiotic resistance worldwide has highlighted the need for new treatments for infections caused by resistant bacteria. Antimicrobial peptides (AMPs) emerge as promising alternatives due to their diverse mechanisms of action. In this context, the AMP AS-48 derived from Enterococcus faecalis, emerges due to its antibacterial properties. This study focused on producing recombinant AS-48 peptide using the green microalga Chlamydomonas reinhardtii. Genetic transformation of C. reinhardtii was successfully achieved with the gene encoding the AS-48 peptide, and a transplastomic line was identified. The presence and quantity of the AS-48 peptide was confirmed through enzyme-linked immunosorbent assay (ELISA), obtaining maximum concentrations of 0.044 μg μL−1. The antimicrobial activity of the total soluble protein (TSP) of the transplastomic C. reinhardtii strain including an untransformed strain was tested against a Gram positive bacterium. The results showed that transplastomic C. reinhardtii TSP exhibits antimicrobial activity against Listeria monocytogenes ATCC 19118. These results suggest that microalgae can be effectively used to produce antimicrobial peptides like AS-48. This approach has the potential to develop new strategies to combat infections caused by antibiotic-resistant bacteria, providing a promising avenue for future research and application in biotechnology.