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<p>This study investigated the use of light-emitting diodes (LEDs) and phytohormones to enhance cell growth and eicosapentaenoic acid accumulation in <i>Pavlova lutheri</i>. A two-phase culture system was employed to optimize biomass and lipid production. In the first phase, focused on biomass enhancement, optimal concentrations of auxins (2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, and 1-naphthaleneacetic acid) and gibberellic acid were evaluated, and photoperiod was also optimized, all under blue LED irradiation. In the second phase, various concentrations of abscisic acid were tested to stimulate lipid accumulation under yellow LED light. Under optimized conditions, the highest biomass yield was achieved using 2,4-dichlorophenoxyacetic acid (5&#xa0;mg L<sup>−1</sup>) combined with gibberellic acid (20&#xa0;mg L<sup>−1</sup>) under a 18:6&#xa0;h light–dark cycle in the blue LED-illuminated first phase. Subsequently, in the yellow LED-illuminated second phase, lipid content reached 50.7% with the addition of 0.5&#xa0;mg L<sup>−1</sup> abscisic acid. Compared to the control, this optimized two-phase treatment with phytohormones resulted in 1.9-fold and 1.4-fold increases in biomass and lipid production, respectively.</p>

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Enhancing cell growth and EPA production in the marine microalga Pavlova lutheri via phytohormone treatment and LED light modulation

  • 期刊论文

  • 发表日期：

    2025年9月3日

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  • So Hee Kim,
  • Ui Hun Lee,
  • Sang Baek Lee,
  • Gwi-Taek Jeong

摘要

This study investigated the use of light-emitting diodes (LEDs) and phytohormones to enhance cell growth and eicosapentaenoic acid accumulation in Pavlova lutheri. A two-phase culture system was employed to optimize biomass and lipid production. In the first phase, focused on biomass enhancement, optimal concentrations of auxins (2,4-dichlorophenoxyacetic acid, indole-3-acetic acid, and 1-naphthaleneacetic acid) and gibberellic acid were evaluated, and photoperiod was also optimized, all under blue LED irradiation. In the second phase, various concentrations of abscisic acid were tested to stimulate lipid accumulation under yellow LED light. Under optimized conditions, the highest biomass yield was achieved using 2,4-dichlorophenoxyacetic acid (5 mg L−1) combined with gibberellic acid (20 mg L−1) under a 18:6 h light–dark cycle in the blue LED-illuminated first phase. Subsequently, in the yellow LED-illuminated second phase, lipid content reached 50.7% with the addition of 0.5 mg L−1 abscisic acid. Compared to the control, this optimized two-phase treatment with phytohormones resulted in 1.9-fold and 1.4-fold increases in biomass and lipid production, respectively.