Scholarlink

<p>The term "eucheumatoids" refers to a group of seaweeds that produce carrageenan, mostly comprising of the well-studied <i>Kappaphycus</i> and <i>Eucheuma</i> genera. The recently reclassified species <i>Kappaphycopsis cottonii</i> (previously <i>Kappaphycus cottonii</i>) is one of the least studied eucheumatoid species. The presence of wild <i>Ka</i>. <i>cottonii</i> in Lombok, Indonesia was molecularly confirmed using mitochondrial markers <i>cox1</i> and <i>cox2-3 s.</i> The carrageenan extracted from wild population measured gel strength of 897 ± 34.56&#xa0;g&#xa0;cm<sup>−2</sup> and viscosity of 76.15 ± 15.23 cP, that may be suitable for specific food or industrial applications. Furthermore, this study encountered cystocarpic female gametophytes and is the first to isolate carpospores from the diminutive carposporophyte life stage attached to the female gametophyte. Spore germination and development was followed for 8&#xa0;weeks. The spore size was approximately 39.50 ± 13.47&#xa0;µm upon release. In the first 1–2&#xa0;weeks, the diameter of <i>Ka. cottonii</i> spores grown up to 57.72 ± 9.01&#xa0;µm. The initial rhizoid growth was first observed after four weeks, with the rhizoidal length reaching up to 474.57 ± 199.38&#xa0;µm. Post the eighth week, the <i>Ka. cottonii</i> spores exhibit considerable growth, averaging 218.58 ± 112.08&#xa0;µm diameter in size. Notably, certain spores had significantly larger diameter, measuring between 500&#xa0;µm to more than 1000&#xa0;µm in length. The sporelings were macroscopically visible after the tenth week. The specific growth rate (SGR % day<sup>−1</sup>) based on spore diameter significantly increased from week 2 (SGR = 0.36 ± 1.39% day<sup>−1</sup>) to week 4 (SGR = 1.46 ± 0.76% day<sup>−1</sup>). The maximum SGR recorded was relatively modest (SGR = 1.94 ± 0.83% day<sup>−1</sup>) following the eighth week. Hence, further optimization of culture conditions (e.g., light intensity, temperature, and medium composition) should be undertaken to potentially enhance the growth rate of the sporelings. Nonetheless, the present study offers significant insights for the advancement of spore culture techniques for producing valuable <i>Ka</i>. <i>cottonii</i> seedlings.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular identification, carpospore development, and carrageenan characteristics of wild eucheumatoid Kappaphycopsis cottonii

  • 期刊论文

  • 发表日期：

    2025年6月20日

查看全文
  • Eka Sunarwidhi Prasedya,
  • Hikmaturrohmi Hikmaturrohmi,
  • Fildzah Zati Hulwani,
  • Satya Reza Faturakhmat,
  • Victor Nikijuluw,
  • Shienna Mae C. Gonzaga,
  • Lourie Ann R. Hinaloc,
  • Phaik-Eem Lim,
  • Michael Y. Roleda

摘要

The term "eucheumatoids" refers to a group of seaweeds that produce carrageenan, mostly comprising of the well-studied Kappaphycus and Eucheuma genera. The recently reclassified species Kappaphycopsis cottonii (previously Kappaphycus cottonii) is one of the least studied eucheumatoid species. The presence of wild Ka. cottonii in Lombok, Indonesia was molecularly confirmed using mitochondrial markers cox1 and cox2-3 s. The carrageenan extracted from wild population measured gel strength of 897 ± 34.56 g cm−2 and viscosity of 76.15 ± 15.23 cP, that may be suitable for specific food or industrial applications. Furthermore, this study encountered cystocarpic female gametophytes and is the first to isolate carpospores from the diminutive carposporophyte life stage attached to the female gametophyte. Spore germination and development was followed for 8 weeks. The spore size was approximately 39.50 ± 13.47 µm upon release. In the first 1–2 weeks, the diameter of Ka. cottonii spores grown up to 57.72 ± 9.01 µm. The initial rhizoid growth was first observed after four weeks, with the rhizoidal length reaching up to 474.57 ± 199.38 µm. Post the eighth week, the Ka. cottonii spores exhibit considerable growth, averaging 218.58 ± 112.08 µm diameter in size. Notably, certain spores had significantly larger diameter, measuring between 500 µm to more than 1000 µm in length. The sporelings were macroscopically visible after the tenth week. The specific growth rate (SGR % day−1) based on spore diameter significantly increased from week 2 (SGR = 0.36 ± 1.39% day−1) to week 4 (SGR = 1.46 ± 0.76% day−1). The maximum SGR recorded was relatively modest (SGR = 1.94 ± 0.83% day−1) following the eighth week. Hence, further optimization of culture conditions (e.g., light intensity, temperature, and medium composition) should be undertaken to potentially enhance the growth rate of the sporelings. Nonetheless, the present study offers significant insights for the advancement of spore culture techniques for producing valuable Ka. cottonii seedlings.