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<p>Oxidative stress is believed to be a key factor in neurodegenerative diseases (NDs) such as Alzheimer’s and Parkinson’s. In this context, sulfated polysaccharides (SPs) from marine seaweed are a promising alternative for treating neuronal damage induced by oxidative stress. This study evaluated the antioxidant potential of SPs-rich samples from the green seaweed <i>Caulerpa cupressoides</i> var. <i>flabellata</i> against hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) in murine neuroblastoma cells (Neuro-2a). The cytotoxicity and protective effect of the samples against H<sub>2</sub>O<sub>2</sub> in Neuro-2a cells were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method. Amongst SPs-rich samples, F2.0 showed antioxidant potential and protective effects against H<sub>2</sub>O<sub>2</sub> in Neuro-2a cells by reducing the levels of reactive oxygen species (ROS), under the tested conditions. Since biomedical applications are envisioned, the toxicity of this sample was further investigated. The F2.0 sample genotoxicity and mutagenicity were evaluated by comet assay and the bacterial reverse mutation assay, respectively. No significant changes were observed in the tail moment and the mutagenicity rate (MR). Furthermore, the F2.0 sample did not induce embryotoxicity and visual-motor response in zebrafish models, since no changes were observed in mortality, malformations, and optomotor response (OMR) in larvae. Therefore, the results of this study may contribute to the further development of a new and safe alternative treatment for NDs.</p>

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Sulfated polysaccharides of Caulerpa cupressoides var. flabellata: An investigation into their antioxidant properties and biocompatibility

  • 期刊论文

  • 发表日期：

    2025年5月20日

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  • Pablo Felipe Ferreira Farias,
  • Victor Moraes Rodrigues,
  • Augusto Monteiro de Souza,
  • Douglas Dourado Oliveira,
  • Ana Carolina Luchiari,
  • Hugo Alexandre Oliveira Rocha,
  • Susana Margarida Gomes Moreira

摘要

Oxidative stress is believed to be a key factor in neurodegenerative diseases (NDs) such as Alzheimer’s and Parkinson’s. In this context, sulfated polysaccharides (SPs) from marine seaweed are a promising alternative for treating neuronal damage induced by oxidative stress. This study evaluated the antioxidant potential of SPs-rich samples from the green seaweed Caulerpa cupressoides var. flabellata against hydrogen peroxide (H2O2) in murine neuroblastoma cells (Neuro-2a). The cytotoxicity and protective effect of the samples against H2O2 in Neuro-2a cells were evaluated by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction method. Amongst SPs-rich samples, F2.0 showed antioxidant potential and protective effects against H2O2 in Neuro-2a cells by reducing the levels of reactive oxygen species (ROS), under the tested conditions. Since biomedical applications are envisioned, the toxicity of this sample was further investigated. The F2.0 sample genotoxicity and mutagenicity were evaluated by comet assay and the bacterial reverse mutation assay, respectively. No significant changes were observed in the tail moment and the mutagenicity rate (MR). Furthermore, the F2.0 sample did not induce embryotoxicity and visual-motor response in zebrafish models, since no changes were observed in mortality, malformations, and optomotor response (OMR) in larvae. Therefore, the results of this study may contribute to the further development of a new and safe alternative treatment for NDs.