<p>In the wild, the natural fluctuations of environmental factors (nutrients and light) are known to induce variations in <i>Palmaria palmata</i> growth and composition. To ensure standardized, high-quality products, land-based tank cultivation is a potential solution. However, further research is required to optimize indoor tank cultivation conditions and understand the impact of environmental parameters on <i>P. palmata</i> composition. Thus, <i>P. palmata</i> were cultivated in indoor tanks under varying conditions of light intensity (50, 100, 200&#xa0;µmol photons m⁻²&#xa0;s⁻¹) for 4&#xa0;weeks and nitrogen enrichment (0, 1, 3&#xa0;mM NO₃⁻) for 5&#xa0;weeks. The specific growth rate (SGR), the chemical composition and the <i>in vitro</i> bioactive potential were investigated. The results suggested that the 200&#xa0;µmol photons m⁻²&#xa0;s⁻¹ irradiance was associated with a higher carbohydrate content (53.01 ± 7.58%) but a lower ash content (24.57 ± 1.13%). Without considering the impact of cultivation weeks, a trend (R² = 0.9859) emerged of an increased SGR with the increasing irradiance (on average 0.33 ± 0.23% day⁻¹, 0.45 ± 0.19% day⁻¹, 0.59 ± 0.32% day⁻¹, respectively, for 50, 100 and 200&#xa0;µmol photons m⁻²&#xa0;s⁻¹). Increasing the nitrogen enrichment did not impact the SGR (0.93 ± 0.07% day⁻¹ on average) but increased protein production (on average 18.51 ± 0.28% and 19.30 ± 0.23%, respectively, for 1 and 3&#xa0;mM) by the seaweed. However, for both cultivation conditions no significant differences were found in the bioactive potential. Thus, tank cultivation parameters affected <i>P. palmata</i> growth and composition, and further research is needed to fully understand this potential in the context of commercial farming, might be considered to produce high-quality food.</p> Graphical Abstract <p></p>