<p>A variety of non-functionalized Brönsted-acidic ionic liquids "BAILs" HNMP-HSO₄, HNMP-H₂PO₄, HNMP-Cl, and choline chloride (ChCl)-based deep eutectic solvents (DESs), specifically choline chloride-oxalic acid (ChCl-OxA) and choline chloride-urea (ChCl-Urea), were used as efficient solvents for the extraction of lipids." from <i>Dunaliella salina</i>. The acidity order of these BAILs was evaluated using the Hammett acidity scale H₀ and pKa values measurement and the results showed a good agreement between the observed Brönsted acidity activity sequence in the lipid extraction yields and the Hammett acidity order determined from UV–visible spectroscopy of BAILs. HNMP-HSO₄ showed the highest acidity level (H₀ (CH₂Cl₂) = 0.58, H₀ (H₂O) = 1.51, pKa = 1.38). The best lipid extraction yields (32 ± 1) were obtained using the deep eutectic solvent ChCl-Urea and HNMP-HSO₄ ionic liquids after 24&#xa0;h, compared to the results from Bligh and Dyer extraction. The total lipid yields of ionic liquids were significantly different at 2&#xa0;h and 24&#xa0;h treatment duration (HNMP-HSO₄, total lipid yields 2&#xa0;h = 28, 24&#xa0;h = 32) however, no significant differences were observed in the lipid extraction yields of DESs (ChCl-Urea, total lipid yields 2&#xa0;h = 34 wt%, 24&#xa0;h = 32 wt%). The oils extracted with ionic liquids (ILs) and deep eutectic solvents (DESs) exhibited lower chlorophyll impurities. After 24&#xa0;h of treatment with HNMP-HSO₄, HNMP-H2PO₄, HNMP-Cl, ChCl-Urea, and ChCl-OxA, the chlorophyll residues measured were 0.007, 0.009, 0.010, 0.10, 0.01, and 0.09&#xa0;μg&#xa0;mg⁻¹ of lipid, respectively. These findings highlight the potential of BAILs and DESs as effective and environmentally friendly solvents for enhancing lipid extraction from microalgae, with significant implications for the production of biofuels and other lipid-based products.</p>