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<p>Marine diatoms are highly valued commercially due to their abundant lipids and bioactive substances. <i>Phaeodactylum tricornutum</i>, a key oil-producing diatom, has faced challenges in large-scale cultivation, primarily due to zooplankton predation. Our previous studies identified that the <i>Pt2015</i> overexpression strain (termed oeT) alters the morphotype of <i>P. tricornutum</i> which demonstrated a majority triradiate morphotype and conferred resistance to zooplankton. In this study, we found that under 60-L culture conditions, the oeT strain was maintained at a stable level (84%). Additionally, the growth of the oeT strain was significantly higher than that of wild type (WT), with a 10% increase in accumulated dry weight. Notably, lipid content in the oeT strain was enhanced by 13.4% relative to WT. Moreover, the contents of fatty acid such as C14:0 and C16:1 are much higher than that of WT. These results suggest that the oeT strain demonstrates considerable advantages for large-scale cultivation of <i>P. tricornutum</i>.</p>

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A genetically modified triradiate strain of Phaeodactylum tricornutum demonstrates considerable advantages in a 60-L photobioreactor

  • 期刊论文

  • 发表日期：

    2025年3月1日

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  • Mengjiao Bao,
  • Wenting Yang,
  • Xin Li,
  • Guihong Yu,
  • Wenhui Gu,
  • Shan Gao,
  • Guangce Wang

摘要

Marine diatoms are highly valued commercially due to their abundant lipids and bioactive substances. Phaeodactylum tricornutum, a key oil-producing diatom, has faced challenges in large-scale cultivation, primarily due to zooplankton predation. Our previous studies identified that the Pt2015 overexpression strain (termed oeT) alters the morphotype of P. tricornutum which demonstrated a majority triradiate morphotype and conferred resistance to zooplankton. In this study, we found that under 60-L culture conditions, the oeT strain was maintained at a stable level (84%). Additionally, the growth of the oeT strain was significantly higher than that of wild type (WT), with a 10% increase in accumulated dry weight. Notably, lipid content in the oeT strain was enhanced by 13.4% relative to WT. Moreover, the contents of fatty acid such as C14:0 and C16:1 are much higher than that of WT. These results suggest that the oeT strain demonstrates considerable advantages for large-scale cultivation of P. tricornutum.