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<p>Optimal seed germination is crucial for achieving high yield, making the percentage of germination and proper establishment of seedlings are of great importance in rapeseed cultivation. Therefore, the use of seed priming and seedling spraying with <i>Ascophyllum nodosum</i> seaweed extract (ANE) is of interest. We examined parameters related to rapeseed germination and changes in the expression pattern of target genes and miRNAs due to the application of <i>A. nodosum</i> extract. The treatment involved using a concentration of 0.1% by volume of algae extract at four levels of time treatment, 0, 10, 15 and 30&#xa0;days with leaves and roots sampled in three biological replicates for each treatment. Results showed a decrease in expression miR156 and the SPL9 gene, miR390 and the GRF8 gene, miR166 and the HDZIP gene, and miR396 and the bHLH gene due to the seaweed extract treatment. The expression of miR164 increased, while the NAC gene expression decreased. In the root tissue, the expression of NAC and HDZIP genes increased over time, while miR164 and miR166 decreased over time. Relative expression of GRF8 and miR390 genes, as well as bHLH and miR396 genes, showed an initial increase followed by decrease. The SPL9 gene expression increased, while its related miRNA (miR156) decreased. In leaf tissue the most negative correlation was observed between miR164 with miR156 and miR396, while the most positive correlation was between miR390 with bHLH, HDZIP and SPL9 genes, and miR166 with GRF8 and bHLH with HDZIP. In root tissue, the most negative correlation was found among miR164, miR166, miR390, miR396, bHLH, GRF8 and SPL9, while the most positive correlation was between SPL9 and GRF8 genes with miR390 and bHLH gene with miR166 and miR396. The results suggest that the application of ANE demonstrate positive effects in seed germination, growth and development and plays functional role in orchestrating miRNA, transcription factors and target genes expression patterns involved in stem apical meristem and root apical meristem.</p>

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Foliar application Ascophyllum nodosum extract biostimulants regulated the stem and root apical meristem by orchestrating miRNA-targets gene transcription and regulatory network in rapeseed (Brassica napus)

  • 期刊论文

  • 发表日期：

    2025年2月13日

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  • Hassan Mohseni,
  • Karim Sorkheh,
  • Daryuosh Nabati Ahmadi

摘要

Optimal seed germination is crucial for achieving high yield, making the percentage of germination and proper establishment of seedlings are of great importance in rapeseed cultivation. Therefore, the use of seed priming and seedling spraying with Ascophyllum nodosum seaweed extract (ANE) is of interest. We examined parameters related to rapeseed germination and changes in the expression pattern of target genes and miRNAs due to the application of A. nodosum extract. The treatment involved using a concentration of 0.1% by volume of algae extract at four levels of time treatment, 0, 10, 15 and 30 days with leaves and roots sampled in three biological replicates for each treatment. Results showed a decrease in expression miR156 and the SPL9 gene, miR390 and the GRF8 gene, miR166 and the HDZIP gene, and miR396 and the bHLH gene due to the seaweed extract treatment. The expression of miR164 increased, while the NAC gene expression decreased. In the root tissue, the expression of NAC and HDZIP genes increased over time, while miR164 and miR166 decreased over time. Relative expression of GRF8 and miR390 genes, as well as bHLH and miR396 genes, showed an initial increase followed by decrease. The SPL9 gene expression increased, while its related miRNA (miR156) decreased. In leaf tissue the most negative correlation was observed between miR164 with miR156 and miR396, while the most positive correlation was between miR390 with bHLH, HDZIP and SPL9 genes, and miR166 with GRF8 and bHLH with HDZIP. In root tissue, the most negative correlation was found among miR164, miR166, miR390, miR396, bHLH, GRF8 and SPL9, while the most positive correlation was between SPL9 and GRF8 genes with miR390 and bHLH gene with miR166 and miR396. The results suggest that the application of ANE demonstrate positive effects in seed germination, growth and development and plays functional role in orchestrating miRNA, transcription factors and target genes expression patterns involved in stem apical meristem and root apical meristem.