<p><i>Porphyridium purpureum</i> is one a promising source of valuable substances. In this research, biomass production and the accumulation of bioactive compounds including phycobiliproteins (PBP), carotenoid, chlorophyll and exopolysaccharides (EPS) in <i>P. purpureum</i> were investigated at different concentrations of NaCl and NaNO₃ and light treatments (white, red and blue) for 5 weeks in F/2 medium. The best biomass production (0.958 g L^-1) was achieved at 50 g L^-1 NaCl after 5 weeks of cultivation. The highest content of all pigments was 64.70 mg L^-1 PBP (48.92 and 11.24 mg L^-1 phycoerythrin (PE) and phycocyanin (PC) respectively), 5.41 mg L^-1 chlorophyll <i>a</i>, and 1.90 mg L^-1 carotenoids after 4 weeks at 50 g L^-1 NaCl and 0.375 g L^-1 NaNO₃. At the same time, the maximum EPS production (70.97 mg L^-1) was achieved in lower concentrations of NaCl (25 g L^-1) and NaNO₃ (0.075 g L^-1). White light acts more efficiently than other lights in biomass (1.23 g L^-1) and the content of all pigments (58.42 mg L^-1 PBP, 5.81 mg L^-1 chlorophyll <i>a</i>, and 1.69 mg L^-1 carotenoids) in 4 weeks. Results showed EPS production needs a stationary phase in which the increase occurs after 4 weeks. Also, red light acts as an inducer for EPS enhancement (78.7 mg L^-1). External (EPS) and internal (biomass and pigments) metabolite production depends on low concentration of nitrate and higher salinity, respectively.</p>