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<p>The yield of recombinant protein production in cyanobacteria remains a bottleneck in the popularization of this biofactory, despite all the advantageous characteristics regarding its use, such as the use of atmospheric CO<sub>2</sub> and solar energy as a carbon source for biomass growth and organic compound synthesis. This study used the cyanobacterium <i>Synechococcus elongatus</i> PCC 7942 for recombinant protein production, determining the best induction parameters of two cyanobacterial photosystem II promoters (PpsbA1 and PpsbA2) and associating these parameters with the application of magnetic fields (MF) and light intensity. Different light intensities (50, 250, and 500&#xa0;μmol photons m⁻<sup>2</sup>&#xa0;s⁻<sup>1</sup>) and exposure times (6, 12, 24, and 48&#xa0;h) were tested in the production of the reporter protein ZsGreen1. The expression of the <i>zsgreen</i> gene and fluorescence levels in transgenic strains were quantified, revealing that the highest expression of this gene was observed in the strain containing the PpsbA2 promoter under high light (500&#xa0;μmol photons m⁻<sup>2</sup>&#xa0;s⁻<sup>1</sup>) for 6&#xa0;h. The best fluorescence results were also obtained at the highest light intensity for this promoter. Furthermore, when a 30 mT MF was applied, <i>zsgreen</i> gene expression and protein fluorescence were even higher with the PpsbA2 promoter, demonstrating the positive effect of this tool in optimizing recombinant protein production. These results suggest magnetic fields as a new tool for improving recombinant protein production in <i>S. elongatus</i> PCC 7942 with the PpsbA2 promoter, as well as highlighting the importance of light intensity and exposure time in the induction of these photosystem II-related promoters.</p> Graphical abstract <p></p>

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Combined effect of light and magnetic field on recombinant protein production driven by photosystem II-related gene promoters in the cyanobacterium Synechococcus elongatus PCC 7942

  • 期刊论文

  • 发表日期：

    2025年1月4日

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  • Arthur C. S. Cardoso,
  • Raíza S. Azevedo,
  • Lucielen O. Santos,
  • Luis F. Marins

摘要

The yield of recombinant protein production in cyanobacteria remains a bottleneck in the popularization of this biofactory, despite all the advantageous characteristics regarding its use, such as the use of atmospheric CO2 and solar energy as a carbon source for biomass growth and organic compound synthesis. This study used the cyanobacterium Synechococcus elongatus PCC 7942 for recombinant protein production, determining the best induction parameters of two cyanobacterial photosystem II promoters (PpsbA1 and PpsbA2) and associating these parameters with the application of magnetic fields (MF) and light intensity. Different light intensities (50, 250, and 500 μmol photons m⁻2 s⁻1) and exposure times (6, 12, 24, and 48 h) were tested in the production of the reporter protein ZsGreen1. The expression of the zsgreen gene and fluorescence levels in transgenic strains were quantified, revealing that the highest expression of this gene was observed in the strain containing the PpsbA2 promoter under high light (500 μmol photons m⁻2 s⁻1) for 6 h. The best fluorescence results were also obtained at the highest light intensity for this promoter. Furthermore, when a 30 mT MF was applied, zsgreen gene expression and protein fluorescence were even higher with the PpsbA2 promoter, demonstrating the positive effect of this tool in optimizing recombinant protein production. These results suggest magnetic fields as a new tool for improving recombinant protein production in S. elongatus PCC 7942 with the PpsbA2 promoter, as well as highlighting the importance of light intensity and exposure time in the induction of these photosystem II-related promoters.

Graphical abstract