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<p><i>Pyropia haitanensis</i>, an economically cultivated red alga, exhibits both sexual and asexual reproduction. Genetic studies have revealed that the gametophytic blades produced by sexual reproduction are monoecious, while those produced by parthenogenesis of female gametophyte and apogamy of male gametophyte are unisexual. However, identifying the sex of <i>P. haitanensis</i> from different sources at the molecular level is still lacking. In this study, female- and male-specific marker primers were used to amplify the genomic DNA of conchocelis and blades from different sources. The results showed that only a female-specific band was amplified in both conchocelis and blades produced by parthenogenesis, while only a male-specific band was amplified in both conchocelis and blades produced by apogamy. In heterozygous conchocelis produced by sexual reproduction and their subsequent offspring four-color-sectored blades, both male and female-specific bands were amplified. Among the wild blades of <i>P. haitanensis</i>, 83.3% of the individuals exhibited both female- and male-specific bands. These findings confirmed the sex of offspring produced by sexual reproduction, parthenogenesis, and apogamy of <i>P. haitanensis</i> at the molecular level.</p>

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Application of sex-specific markers in identifying the sex of Pyropia haitanensis from different sources

  • 期刊论文

  • 发表日期：

    2025年1月2日

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  • Hong-Chang Ding,
  • Hui-Ping Ma,
  • Long-Chen Tang,
  • Xing‑Hong Yan

摘要

Pyropia haitanensis, an economically cultivated red alga, exhibits both sexual and asexual reproduction. Genetic studies have revealed that the gametophytic blades produced by sexual reproduction are monoecious, while those produced by parthenogenesis of female gametophyte and apogamy of male gametophyte are unisexual. However, identifying the sex of P. haitanensis from different sources at the molecular level is still lacking. In this study, female- and male-specific marker primers were used to amplify the genomic DNA of conchocelis and blades from different sources. The results showed that only a female-specific band was amplified in both conchocelis and blades produced by parthenogenesis, while only a male-specific band was amplified in both conchocelis and blades produced by apogamy. In heterozygous conchocelis produced by sexual reproduction and their subsequent offspring four-color-sectored blades, both male and female-specific bands were amplified. Among the wild blades of P. haitanensis, 83.3% of the individuals exhibited both female- and male-specific bands. These findings confirmed the sex of offspring produced by sexual reproduction, parthenogenesis, and apogamy of P. haitanensis at the molecular level.