A novel collaborative signal amplification biosensor based on sandwich-type composite film for ultrasensitive detection of rhEPO
摘要
This research established a highly sensitive and low-cost electrochemical biosensor for the detection of recombinant human erythropoietin. A sandwich-like gold nanoparticles/Chitosan/gold nanoparticles composite film was prepared on a glassy carbon electrode. The gold nanoparticles/Chitosan/gold nanoparticles film, comprising gold nanoparticles with a large specific surface area, high conductivity, and excellent biocompatibility, was paired with Chitosan, which was naturally high in content, biodegradable, and biocompatible. Immobilization of the erythropoietin receptor on gold nanoparticles/Chitosan/gold nanoparticles films allowed recombinant human erythropoietin to recognize erythropoietin receptor, thereby altering the number of pores. The electrochemical results demonstrated that the gold nanoparticles/Chitosan/gold nanoparticle-modified electrochemical biosensor exhibited a broad detection range (5 × 10–13 to 5 × 10–9 g L−1) and a low detection limit (5.16 × 10–14 g L−1). The overall preparation process was straightforward, and the detection process was rapid, with an incubation period of only 20 min. Furthermore, the biosensor exhibited high specificity, repeatability, and stability. In conclusion, the electrochemical biosensor was a simple and rapid method for the specific and accurate detection of trace recombinant human erythropoietin, which was suitable for clinical monitoring and the identification of doping.
Graphical abstract