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Purpose <p>Compare optical properties of amniotic membranes (AM) using a modified photographic-based method.</p> Methods <p>Optical measures of various AMs (Prokera® Plus, Prokera® Slim, Amniograft®, Ambio2®, Ambio5®) and a Collagen Shield were quantified by examining standardized band pattern images through the AMs. Blur Index (BI) was calculated by comparing standard deviations of gray values, while transparency ratio (TR) was determined by comparing mean peak gray values of white bars viewed through the AMs.</p> Results <p>BIs ranged from 0.97% (Ambio2®) to 22.58% (Ambio5®), with 0% signifying minimal blurring. TRs ranged between 0.906 to 1.018, with greater values signifying more brightness. BI and TR showed an inverse correlation (r = −&#xa0;0.954, 95% CI −&#xa0;0.984, −&#xa0;0.871). Thicker AMs generally demonstrated higher BIs. Cross-linking effects were unpredictable.</p> Conclusion <p>TR inversely correlated with BI and thicker AMs generally exhibited higher BIs. This method offers a rapid, cost-effective approach to comparing AM optical qualities, providing insights into patient vision.</p>

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Comparative analysis of optical properties in amniotic membrane products

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月13日

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  • Bryanna J. Lee,
  • Jeffrey Tsao,
  • Natalie A. Afshari

摘要

Purpose

Compare optical properties of amniotic membranes (AM) using a modified photographic-based method.

Methods

Optical measures of various AMs (Prokera® Plus, Prokera® Slim, Amniograft®, Ambio2®, Ambio5®) and a Collagen Shield were quantified by examining standardized band pattern images through the AMs. Blur Index (BI) was calculated by comparing standard deviations of gray values, while transparency ratio (TR) was determined by comparing mean peak gray values of white bars viewed through the AMs.

Results

BIs ranged from 0.97% (Ambio2®) to 22.58% (Ambio5®), with 0% signifying minimal blurring. TRs ranged between 0.906 to 1.018, with greater values signifying more brightness. BI and TR showed an inverse correlation (r = − 0.954, 95% CI − 0.984, − 0.871). Thicker AMs generally demonstrated higher BIs. Cross-linking effects were unpredictable.

Conclusion

TR inversely correlated with BI and thicker AMs generally exhibited higher BIs. This method offers a rapid, cost-effective approach to comparing AM optical qualities, providing insights into patient vision.