<p>This experimental study discusses the stability and thermophysical properties of a 2 wt% TiO₂–water nanofluid with the addition of different low-molecular-weight and macromolecular ionic and non-ionic surfactants. The main aim is to draw attention to the surfactant impact on thermophysical properties and what are the advantages and disadvantages of employing a certain dispersant. Significant gaps persist regarding the specific influence of surfactants on these systems and their subsequent thermophysical behavior. This experimental analysis provides a comprehensive understanding of TiO₂ water nanofluids enriched with several surfactants of different categories: commercial, tailor-made, and industrial dispersants. To address these, an analysis was achieved in terms of stability, morphology, and thermophysical properties of 14 samples of different concentrations. Results revealed that the thermal conductivity is lowly influenced by the addition of considered surfactants, which is beneficial for further heat-transfer applications. On the other hand, the viscosity is affected by larger concentrations of dispersants, as well as the polydispersity index as determined by DLS. As a conclusion, adding surfactants has to be carefully considered and a prior analysis in terms of benefits and drawbacks is mandatory.</p>