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<p>Extreme temperature fluctuations present substantial challenges to fish survival and reproductive success. <i>Perccottus glenii</i>, a cold-tolerant freshwater species, has emerged as a valuable model for elucidating the molecular mechanisms underlying cold adaptation. Although alternative splicing (AS) is recognized as a crucial post-transcriptional regulatory mechanism, its specific role in cold tolerance remains inadequately characterized. In this study, we conducted a comprehensive transcriptomic analysis of AS events in the brain, liver, and muscle tissues of <i>P. glenii</i> across three physiological states: active, freezing, and recovery. A large number of differentially alternatively spliced genes (DASGs) and differentially expressed genes (DEGs) were identified. Gene Ontology (GO) enrichment analysis revealed distinct tissue-specific functional patterns. Among the three tissues, the enrichment results of DASGs and DEGs revealed both overlapping and distinct functional categories, suggesting that both mechanisms act in concert to facilitate cold adaptation. Notably, splicing variants of key genes such as <i>adgrb1a</i>, <i>baxa,</i> and <i>stat3</i> were identified as potential contributors to cold adaptation. Collectively, these findings underscore the pivotal role of alternative splicing in the cold adaptation of <i>P. glenii</i> and offer a theoretical foundation for enhancing cold resistance in aquaculture and cryopreservation applications.</p>

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Alternative splicing mechanisms contributing to cold adaptation in the Amur sleeper (Perccottus glenii)

  • 期刊论文

  • 发表日期：

    2026年6月16日

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  • Huihua Lu,
  • Wenhao Li,
  • Zhengchao Zhu,
  • Peng Hu,
  • Liangbiao Chen,
  • Qianghua Xu

摘要

Extreme temperature fluctuations present substantial challenges to fish survival and reproductive success. Perccottus glenii, a cold-tolerant freshwater species, has emerged as a valuable model for elucidating the molecular mechanisms underlying cold adaptation. Although alternative splicing (AS) is recognized as a crucial post-transcriptional regulatory mechanism, its specific role in cold tolerance remains inadequately characterized. In this study, we conducted a comprehensive transcriptomic analysis of AS events in the brain, liver, and muscle tissues of P. glenii across three physiological states: active, freezing, and recovery. A large number of differentially alternatively spliced genes (DASGs) and differentially expressed genes (DEGs) were identified. Gene Ontology (GO) enrichment analysis revealed distinct tissue-specific functional patterns. Among the three tissues, the enrichment results of DASGs and DEGs revealed both overlapping and distinct functional categories, suggesting that both mechanisms act in concert to facilitate cold adaptation. Notably, splicing variants of key genes such as adgrb1a, baxa, and stat3 were identified as potential contributors to cold adaptation. Collectively, these findings underscore the pivotal role of alternative splicing in the cold adaptation of P. glenii and offer a theoretical foundation for enhancing cold resistance in aquaculture and cryopreservation applications.