<p>Glacier surfaces and their prominent ecosystems in the cryoconite holes harbor a broad spectrum of organisms. The diversity of metazoans is relatively low, which is probably due to the particular pressure that environment imposes on its inhabitants. In particular, nematodes, which are widespread and ubiquitous in most environments, have previously been reported as absent from cryoconite holes worldwide. Here we report a population of the family Monhysteridae from the cryoconite holes of the Lenangsbreen glacier in northern Norway. The nematodes were characterized by a combination of morphological taxonomy and DNA sequencing. All specimens examined were female, some with eggs in the gonad. All showed similar morphological features and almost identical LSU sequences. They reached 0–118 individuals g⁻¹ of dry sediment and 60% permanence, which is less than rotifers and tardigrades found in the same holes. Altogether 17 specimens have been found in 6 cryoconite holes during sampling. No significant relation was found between the nematodes and sediment parameters, nitrogen or organic and inorganic carbon in the holes. Our findings suggest that the nematode population was not an exceptional case of single nematode individuals on the glacier, but formed in cryoconite holes a uniform and reproducing lineage.</p>