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<p>The stress urinary incontinence (SUI) is a difficulty in urology and current sub-urethral sling treatments are associated with inflamation and recurrence. In this study, we developed a novel tissue-engineered sling with myogenic induced adiposederived stem cells (MI-ADSCs) sheets induced by 5-Aza and combined with electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) (SF/PLGA) for the treatment of stress urinary incontinence. MI-ADSCs increased α-SMA, MyoD and Desmin the mRNA and protein expression. ICG001, a specific inhibitor of β-catenin signaling pathway, could inhibit 5-Aza-induced expression of myoblast genes and proteins. Electrospun scaffolds of SF/PLGA (50/50) showed favorable mechanical properties and the biocompatibility. The MI-ADSCs sheets was combined with electrospun scaffolds of SF/PLGA (50/50) to form a novel tissue-engineered sling. The tissue-engineered sling was transplanted into female SUI rats to evaluate the feasibility and effcacy for the treatment of SUI. The tissue-engineered sling with MI-ADSCs sheets combined with SF/PLGA can restore leak point pressure (LPP) in the model of SUI steadily. It is of great potential to be a novel tissue-engineered sling in the treatment of SUI.</p>

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Myogenic induced adipose-derived stem cells sheets combined with electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) for stress urinary incontinence treatment

  • 期刊论文

  • 发表日期：

    2025年9月10日

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  • Ming Liu,
  • Yongwei Li,
  • Yining Zhao,
  • Fengze Sun,
  • Youyi Lu,
  • Jitao Wu,
  • Qingsong Zou

摘要

The stress urinary incontinence (SUI) is a difficulty in urology and current sub-urethral sling treatments are associated with inflamation and recurrence. In this study, we developed a novel tissue-engineered sling with myogenic induced adiposederived stem cells (MI-ADSCs) sheets induced by 5-Aza and combined with electrospun scaffolds of silk fibroin and poly(lactide-co-glycolide) (SF/PLGA) for the treatment of stress urinary incontinence. MI-ADSCs increased α-SMA, MyoD and Desmin the mRNA and protein expression. ICG001, a specific inhibitor of β-catenin signaling pathway, could inhibit 5-Aza-induced expression of myoblast genes and proteins. Electrospun scaffolds of SF/PLGA (50/50) showed favorable mechanical properties and the biocompatibility. The MI-ADSCs sheets was combined with electrospun scaffolds of SF/PLGA (50/50) to form a novel tissue-engineered sling. The tissue-engineered sling was transplanted into female SUI rats to evaluate the feasibility and effcacy for the treatment of SUI. The tissue-engineered sling with MI-ADSCs sheets combined with SF/PLGA can restore leak point pressure (LPP) in the model of SUI steadily. It is of great potential to be a novel tissue-engineered sling in the treatment of SUI.