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<p>MYB transcription factors play pivotal roles in anthocyanin biosynthesis and its regulatory network. In this study, <i>CsMYB90</i> was cloned and heterologously expressed in tobacco (<i>Nicotiana tabacum</i> cv. K326), followed by systematic functional validation through integrated phenotypic, transcriptomic, and metabolomic analyses. The results showed that transgenic tobacco leaves exhibited a striking purple phenotype, with anthocyanin accumulation reached 13.04-fold higher than wild-type control. <i>CsMYB90</i> significantly elevated the levels of anthocyanin pigments, specifically delphinidin-3-O-rhamnoside (783.14&#xa0;µg/g) and cyanidin-3-O-glucoside (209.40&#xa0;µg/g), by promoting the expression of key structural genes such as <i>dihydroflavonol reductase</i> (<i>DFR</i>), <i>chalcone isomerase</i> (<i>CHI</i>), and <i>phenylalanine ammonia-lyase</i> (<i>PAL</i>) thereby inducing the purple phenotype. Furthermore, we preliminarily confirmed that <i>CsMYB90</i> has transcriptional activation activity on the downstream gene <i>NtDFR1</i> of anthocyanins, and the overexpression of <i>CsMYB90</i> enhanced the cold tolerance of transgenic tobacco. These findings enrich the theoretical basis of MYBs regulation of anthocyanins, and provide data support for the application of <i>CsMYB90</i> in plant anthocyanin regulation molecular breeding.</p>

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Overexpression of CsMYB90 enhances anthocyanin accumulation and alters metabolic pathways in transgenic tobacco

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年11月20日

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  • Yin You,
  • Ju Cai,
  • Sumeera Asghar,
  • Xiuliu Yang,
  • Litang Lv,
  • Xiaofang Zeng,
  • Jianrong Li,
  • Qinglin Liu,
  • Yan Li

摘要

MYB transcription factors play pivotal roles in anthocyanin biosynthesis and its regulatory network. In this study, CsMYB90 was cloned and heterologously expressed in tobacco (Nicotiana tabacum cv. K326), followed by systematic functional validation through integrated phenotypic, transcriptomic, and metabolomic analyses. The results showed that transgenic tobacco leaves exhibited a striking purple phenotype, with anthocyanin accumulation reached 13.04-fold higher than wild-type control. CsMYB90 significantly elevated the levels of anthocyanin pigments, specifically delphinidin-3-O-rhamnoside (783.14 µg/g) and cyanidin-3-O-glucoside (209.40 µg/g), by promoting the expression of key structural genes such as dihydroflavonol reductase (DFR), chalcone isomerase (CHI), and phenylalanine ammonia-lyase (PAL) thereby inducing the purple phenotype. Furthermore, we preliminarily confirmed that CsMYB90 has transcriptional activation activity on the downstream gene NtDFR1 of anthocyanins, and the overexpression of CsMYB90 enhanced the cold tolerance of transgenic tobacco. These findings enrich the theoretical basis of MYBs regulation of anthocyanins, and provide data support for the application of CsMYB90 in plant anthocyanin regulation molecular breeding.