Scholarlink

<p>The sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family of protein kinases plays a crucial role in plant salt stress tolerance. To investigate the function of the <i>BvSnRK2.1</i> gene in the response to salt stress in sugar beet (<i>Beta vulgaris</i> L.), the full-length coding sequence (CDS) of <i>BvSnRK2.1</i> was cloned, and the transgenic tobacco lines overexpressing <i>BvSnRK2.1</i> were generated. According to the findings, transgenic tobacco lines with overexpression of <i>BvSnRK2.1</i> gene from sugar beet showed improved root system development, increased levels of proline, sucrose, soluble protein, total soluble sugars and chlorophyll, increased activities of antioxidant enzymes (e.g., peroxidase, catalase and superoxide dismutase) and improved photosynthetic efficiency in saline conditions. Additionally, genes related to proline synthesis (<i>NbP5CR</i>), ion transport (<i>NbSOS1</i>, <i>NbNHX</i>, and <i>NbAKT</i>), and reactive oxygen species scavenging (e.g., <i>NbCAT</i>, <i>NbSOD</i>, and <i>NbPOD</i>) were more highly expressed in tobacco plants overexpressing <i>BvSnRK2.1</i>. In short, <i>BvSnRK2.1</i> coordinated several defense mechanisms, such as ion homeostasis, osmoregulation and antioxidant, to greatly improve plant response to salt stress.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Overexpression of BvSnRK2.1, a novel gene encoding the sucrose non-fermenting 1-related protein kinase 2 from sugar beet (Beta vulgaris L.), confers salt tolerance in transgenic tobacco

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月9日

查看全文
  • Xin-Miao Zhang,
  • Jing-Min Ren,
  • Guo-Qiang Wu,
  • Ming Wei,
  • Bin Cheng

摘要

The sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family of protein kinases plays a crucial role in plant salt stress tolerance. To investigate the function of the BvSnRK2.1 gene in the response to salt stress in sugar beet (Beta vulgaris L.), the full-length coding sequence (CDS) of BvSnRK2.1 was cloned, and the transgenic tobacco lines overexpressing BvSnRK2.1 were generated. According to the findings, transgenic tobacco lines with overexpression of BvSnRK2.1 gene from sugar beet showed improved root system development, increased levels of proline, sucrose, soluble protein, total soluble sugars and chlorophyll, increased activities of antioxidant enzymes (e.g., peroxidase, catalase and superoxide dismutase) and improved photosynthetic efficiency in saline conditions. Additionally, genes related to proline synthesis (NbP5CR), ion transport (NbSOS1, NbNHX, and NbAKT), and reactive oxygen species scavenging (e.g., NbCAT, NbSOD, and NbPOD) were more highly expressed in tobacco plants overexpressing BvSnRK2.1. In short, BvSnRK2.1 coordinated several defense mechanisms, such as ion homeostasis, osmoregulation and antioxidant, to greatly improve plant response to salt stress.