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<p>One of the key regulators of pollen germination on the stigma are reactive oxygen species (ROS). It is known that pollen germination of many plant species is sensitive to them, and ROS production on the stigma is typical for angiosperms. However, the balance between two major ROS, H<sub>2</sub>O<sub>2</sub> and О<sup>•</sup><sub>2</sub>¯, is species-specific. It has been studied in representatives of several dicotyledonous families, while for monocotyledons such data were critically few. Using a complex method, including EPR spectroscopy and spectrophotometry, the production of total ROS, hydrogen peroxide, and superoxide radical in the stigma exudate of monocotyledonous plants from three families were measured for the first time. Total ROS production on stigmas of plants from Bromeliaceae family was 10–50 times greater than that of hyacinth (Asparagaceae), and 2 orders of magnitude higher than that in Orchidaceae. In the stigma exudate of bromeliads, the concentration of H<sub>2</sub>O<sub>2</sub> is moderate and the level of О<sup>•</sup><sub>2</sub>¯ generation is high, indicating similarity with a previously studied representative of Liliaceae. Despite the predominance of superoxide radical in the stigma exudate, H<sub>2</sub>O<sub>2</sub> effectively stimulates pollen germination of three bromeliad species in vitro. In orchids and hyacinth, О<sup>•</sup><sub>2</sub>¯ was not detected, and hydrogen peroxide concentrations were low. Thus, representatives of two orders, Poales and Asparagales, are characterized by different patterns of ROS generation, and these patterns reveal their similarity with other families of angiosperms and with gymnosperms.</p>

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Which reactive oxygen species are produced on wet stigmas of different monocot plants?

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  • 发表日期：

    2025年5月13日

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  • Maria Breygina,
  • Oksana Luneva,
  • Ksenia Babushkina,
  • Maria Kushunina,
  • Nadezhda Lazareva,
  • Ekaterina Klimenko

摘要

One of the key regulators of pollen germination on the stigma are reactive oxygen species (ROS). It is known that pollen germination of many plant species is sensitive to them, and ROS production on the stigma is typical for angiosperms. However, the balance between two major ROS, H2O2 and О•2¯, is species-specific. It has been studied in representatives of several dicotyledonous families, while for monocotyledons such data were critically few. Using a complex method, including EPR spectroscopy and spectrophotometry, the production of total ROS, hydrogen peroxide, and superoxide radical in the stigma exudate of monocotyledonous plants from three families were measured for the first time. Total ROS production on stigmas of plants from Bromeliaceae family was 10–50 times greater than that of hyacinth (Asparagaceae), and 2 orders of magnitude higher than that in Orchidaceae. In the stigma exudate of bromeliads, the concentration of H2O2 is moderate and the level of О•2¯ generation is high, indicating similarity with a previously studied representative of Liliaceae. Despite the predominance of superoxide radical in the stigma exudate, H2O2 effectively stimulates pollen germination of three bromeliad species in vitro. In orchids and hyacinth, О•2¯ was not detected, and hydrogen peroxide concentrations were low. Thus, representatives of two orders, Poales and Asparagales, are characterized by different patterns of ROS generation, and these patterns reveal their similarity with other families of angiosperms and with gymnosperms.