Scholarlink

<p>Low-light has emerged as a primary environmental factor limiting rice production, yet there is currently limited research on relevant regulatory measures. In this study, a low-light-intolerant <i>RGA1</i> gene mutant <i>d1</i>, and its wild type (Zhonghua 11, WT), along with <i>RGA1</i> overexpression line OE-1, were used to investigate the effects of nitrogen levels on rice yield formation under low-light condition during anthesis, using field shading treatment. Our results showed that low-light significantly decreased spikelet fertility, with inhibited pollen tube elongation leading to spikelet abortion. We found that RGA1 could promote pollen tube elongation resulted from low-light stress at low nitrogen (60&#xa0;kg N·ha<sup>− 1</sup>, LN) and medium nitrogen (160&#xa0;kg N·ha<sup>− 1</sup>, MN) levels. Under low-light condition, the MN treatment notably increased the ratio of pollen tube entering into the ovule and spikelet fertility compared to the LN treatment, while the high nitrogen treatment (260&#xa0;kg N·ha<sup>− 1</sup>, HN) decreased spikelet fertility and yield in WT and OE-1 plants. For the <i>d1</i> mutant, except for the LN treatment, other nitrogen treatments had minimal effects on spikelet fertility and yield. Furthermore, compared to the LN treatment, the activities of invertase and sucrose synthase, as well as the content of ATP, and ATPase in the spikelets of WT and OE-1 significantly increased when treated with MN under low-light condition. In conclusion, moderately increasing nitrogen levels can enhance sucrose metabolism, maintain energy balance thus alleviating inhibition of pollen tube elongation resulted from low-light stress and improving spikelet fertility.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Nitrogen regulates pollen tube elongation under low-light stress during anthesis to prevent spikelet abortion in rice

  • 期刊论文

  • 发表日期：

    2024年12月31日

查看全文
  • Yichang Zhong,
  • Hubo Li,
  • Feifei Li,
  • Guanfu Fu,
  • Qiao Deng,
  • Dali Zeng,
  • Linzhou Huang

摘要

Low-light has emerged as a primary environmental factor limiting rice production, yet there is currently limited research on relevant regulatory measures. In this study, a low-light-intolerant RGA1 gene mutant d1, and its wild type (Zhonghua 11, WT), along with RGA1 overexpression line OE-1, were used to investigate the effects of nitrogen levels on rice yield formation under low-light condition during anthesis, using field shading treatment. Our results showed that low-light significantly decreased spikelet fertility, with inhibited pollen tube elongation leading to spikelet abortion. We found that RGA1 could promote pollen tube elongation resulted from low-light stress at low nitrogen (60 kg N·ha− 1, LN) and medium nitrogen (160 kg N·ha− 1, MN) levels. Under low-light condition, the MN treatment notably increased the ratio of pollen tube entering into the ovule and spikelet fertility compared to the LN treatment, while the high nitrogen treatment (260 kg N·ha− 1, HN) decreased spikelet fertility and yield in WT and OE-1 plants. For the d1 mutant, except for the LN treatment, other nitrogen treatments had minimal effects on spikelet fertility and yield. Furthermore, compared to the LN treatment, the activities of invertase and sucrose synthase, as well as the content of ATP, and ATPase in the spikelets of WT and OE-1 significantly increased when treated with MN under low-light condition. In conclusion, moderately increasing nitrogen levels can enhance sucrose metabolism, maintain energy balance thus alleviating inhibition of pollen tube elongation resulted from low-light stress and improving spikelet fertility.