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<p>Peruvian lilies (<i>Alstroemeria</i> spp. or cvs.) are regarded as new popular flowers when compared with roses, tulips, lilies, or daffodils, and thus breeders hope to create more cultivars to meet the need for business. To explore efficient ways in breeding new <i>Alstroemeria</i> cultivars, we investigated 20 cultivars using the fluorescence in situ hybridization (FISH) technique in this research. Among the 20 cultivars, there was one diploid (2<i>n</i> = 2<i>x</i> = 16), 12 allotriploids (2<i>n</i> = 3<i>x</i> = 24) except 1 near allotriploid (2<i>n</i> = 3<i>x</i> + 1 = 25), and 7 allotetraploids (2<i>n</i> = 4<i>x</i> = 32). According to FISH analysis in which the tandem repeats; i.e., A001-I (Y10977.1), F3 (AY138115.1) and D032-18 (AJ228817.1), and 45S rDNA (NR_141643.1, NR_141642.1&#xa0;and X52320.1) and 5S rDNA (AJ307346.2), were used as probes, it was deduced that all allotriploids consisted of one genome of <i>A. aurea</i>, one genome of <i>A. psittacina</i>, and one genome of <i>A. pelegrina</i>, or their corresponding close species; and that all seven allotetraploids contained two genomes of <i>A. pelegrina</i> and two genomes of <i>A. psittacina</i>, or their corresponding closely related species. Based on the present results and discussion, it was concluded that <i>A. aurea</i>, <i>A. psittacina</i>, and <i>A. pelegrina</i>, or their corresponding close species were the source of the present cultivars, meanwhile, 2<i>n</i> gametes played the key role in breeding them. Hence, polyploidy would be the main way in breeding new <i>Alstroemeria</i> cultivars.</p>

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Unveiling the genomic composition and breeding origins of Alstroemeria cultivars using fluorescence in situ hybridization (FISH)

  • 期刊论文

  • 发表日期：

    2026年5月2日

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  • Xiaoyan Zhang,
  • Ruijie Ruan,
  • Jia Liu,
  • Lü Zou,
  • Yanni Sun,
  • Shujun Zhou

摘要

Peruvian lilies (Alstroemeria spp. or cvs.) are regarded as new popular flowers when compared with roses, tulips, lilies, or daffodils, and thus breeders hope to create more cultivars to meet the need for business. To explore efficient ways in breeding new Alstroemeria cultivars, we investigated 20 cultivars using the fluorescence in situ hybridization (FISH) technique in this research. Among the 20 cultivars, there was one diploid (2n = 2x = 16), 12 allotriploids (2n = 3x = 24) except 1 near allotriploid (2n = 3x + 1 = 25), and 7 allotetraploids (2n = 4x = 32). According to FISH analysis in which the tandem repeats; i.e., A001-I (Y10977.1), F3 (AY138115.1) and D032-18 (AJ228817.1), and 45S rDNA (NR_141643.1, NR_141642.1 and X52320.1) and 5S rDNA (AJ307346.2), were used as probes, it was deduced that all allotriploids consisted of one genome of A. aurea, one genome of A. psittacina, and one genome of A. pelegrina, or their corresponding close species; and that all seven allotetraploids contained two genomes of A. pelegrina and two genomes of A. psittacina, or their corresponding closely related species. Based on the present results and discussion, it was concluded that A. aurea, A. psittacina, and A. pelegrina, or their corresponding close species were the source of the present cultivars, meanwhile, 2n gametes played the key role in breeding them. Hence, polyploidy would be the main way in breeding new Alstroemeria cultivars.