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<p>Phosphorus (P) deficiency is one of the major constraints to rice productivity due to low soil P availability and poor uptake efficiency in most cultivars. Approximately 70% of global cultivated land is affected by P deficiency. Therefore, developing rice cultivars with improved performance under resource-limited conditions is a key breeding objective. The <i>Phosphorus Starvation Tolerance 1</i> (<i>PSTOL1</i>) gene plays a central role in regulating early root growth, grain yield, and phosphorus acquisition under P-deficient conditions. However, the allelic variation in <i>PSTOL1</i> in wild rice species remains poorly explored. In the present study, superior <i>PSTOL1</i> haplotypes from <i>Oryza rufipogon</i> were introgressed into the elite rice cultivar Punjab Rice 114 (PR114) through marker-assisted selection (MAS). Three donor accessions (IRGC104395, IRGC104852, and IRGC81989) carrying distinct haplotypes were crossed with PR114. <i>PSTOL1</i>-specific markers were used to screen a total of 1764 backcross progenies. Among these, foreground selection identified 468 <i>PSTOL1</i>-positive plants. Subsequent background selection confirmed 72 homozygous BC₂F₂ lines with 69–79% recurrent parent genome recovery. The selected introgression lines (4928–5, 4849–60, 4847–75, 4850–13, and 4849–96) exhibited improved spikelets per panicle under P-deficient conditions. These results demonstrate the introgression of <i>PSTOL1</i> haplotypes from <i>O. rufipogon</i> into PR114 through MAS and provide promising pre-breeding resources for developing phosphorus-use-efficient rice cultivars adapted to low-input and nutrient-deficient ecosystems.</p>

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Marker-assisted introgression of superior haplotypes of Phosphorus Starvation Tolerance 1 (PSTOL1) gene from Oryza rufipogon imparts enhanced phosphorus uptake efficiency

  • 期刊论文

  • 发表日期：

    2026年5月3日

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  • Sahil Jain,
  • Manbir Kaur,
  • Kishor Kumar,
  • Palvi Malik,
  • Salwinder Singh Dhaliwal,
  • Yogesh Vikal,
  • Kuldeep Singh,
  • Kumari Neelam

摘要

Phosphorus (P) deficiency is one of the major constraints to rice productivity due to low soil P availability and poor uptake efficiency in most cultivars. Approximately 70% of global cultivated land is affected by P deficiency. Therefore, developing rice cultivars with improved performance under resource-limited conditions is a key breeding objective. The Phosphorus Starvation Tolerance 1 (PSTOL1) gene plays a central role in regulating early root growth, grain yield, and phosphorus acquisition under P-deficient conditions. However, the allelic variation in PSTOL1 in wild rice species remains poorly explored. In the present study, superior PSTOL1 haplotypes from Oryza rufipogon were introgressed into the elite rice cultivar Punjab Rice 114 (PR114) through marker-assisted selection (MAS). Three donor accessions (IRGC104395, IRGC104852, and IRGC81989) carrying distinct haplotypes were crossed with PR114. PSTOL1-specific markers were used to screen a total of 1764 backcross progenies. Among these, foreground selection identified 468 PSTOL1-positive plants. Subsequent background selection confirmed 72 homozygous BC₂F₂ lines with 69–79% recurrent parent genome recovery. The selected introgression lines (4928–5, 4849–60, 4847–75, 4850–13, and 4849–96) exhibited improved spikelets per panicle under P-deficient conditions. These results demonstrate the introgression of PSTOL1 haplotypes from O. rufipogon into PR114 through MAS and provide promising pre-breeding resources for developing phosphorus-use-efficient rice cultivars adapted to low-input and nutrient-deficient ecosystems.