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<p><i>Limnanthes douglasii</i> is an economically and ecologically significant species, which is valued for its ornamental appeal, pollinator support, and unique seed oil. However, no complete mitochondrial genome has been reported to date. Here, we assembled and annotated its mitochondrial genome using PacBio Sequel II long-read sequencing. The total genome length was 329,140&#xa0;bp, with GC content of 45.55%. The genome contained 36 protein-coding genes (PCGs), 22 tRNAs, and 3 rRNAs. Codon usage analysis revealed significant bias, particularly for arginine, leucine, and serine. We identified 67 simple sequence repeats (SSRs), 32 tandem repeats, and 48 dispersed repeats. Notably, 10 chloroplast-derived DNA fragments were detected, harboring 10 functional genes. We predicted 436 C–U RNA editing sites, predominantly in <i>nad4</i>. Phylogenetic analysis showed <i>L. douglasii</i> grouped within the order Brassicales, consistent with existing taxonomic classifications further. This annotated mitochondrial genome provides a valuable genomic resource for <i>L. douglasii</i> and may support future comparative and evolutionary research in Brassicales.</p>

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Deciphering the chromosomal mitochondrial genome of Limnanthes douglasii

  • 期刊论文

  • 发表日期：

    2026年3月12日

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  • Wang Dong,
  • Jia-Yi Wu,
  • Jia-Yu Xue,
  • Tian-Qi Zhang,
  • Guang-Can Zhou

摘要

Limnanthes douglasii is an economically and ecologically significant species, which is valued for its ornamental appeal, pollinator support, and unique seed oil. However, no complete mitochondrial genome has been reported to date. Here, we assembled and annotated its mitochondrial genome using PacBio Sequel II long-read sequencing. The total genome length was 329,140 bp, with GC content of 45.55%. The genome contained 36 protein-coding genes (PCGs), 22 tRNAs, and 3 rRNAs. Codon usage analysis revealed significant bias, particularly for arginine, leucine, and serine. We identified 67 simple sequence repeats (SSRs), 32 tandem repeats, and 48 dispersed repeats. Notably, 10 chloroplast-derived DNA fragments were detected, harboring 10 functional genes. We predicted 436 C–U RNA editing sites, predominantly in nad4. Phylogenetic analysis showed L. douglasii grouped within the order Brassicales, consistent with existing taxonomic classifications further. This annotated mitochondrial genome provides a valuable genomic resource for L. douglasii and may support future comparative and evolutionary research in Brassicales.