Scholarlink

<p>Enset (<i>Ensete ventricosum</i>) is a crucial crop in Ethiopia, providing food security for rural communities. Despite its significance for nutrition and income, enset is often undervalued and underutilized. The research aimed to evaluate the genetic diversity, population structure, and relationships of these enset landraces. A recent study focused on genotyping 192 autochthonous enset accessions collected from various regions in Southern Ethiopia. The study evaluated the effectiveness of Single-nucleotide polymorphisms (SNPs) markers using tGBS (tunable genotyping by-sequencing) genotyping technology with the Bsp1286I restriction enzyme. Libraries were sequenced, quality-trimmed, and aligned to the <i>Ensete ventricosum</i> reference genome. SNP calling identified 129,649 high-quality markers from 788,103,162 reads generated from tGBS-sequences. These markers were used for the diversity analysis. relationships among regional accessions. The analysis of molecular variance (AMOVA) revealed minimal genetic differentiation among regional accessions 0.6%, with 99.4% of the genetic variation within accessions. Phylogenetic analysis grouped the regional enset accessions into three clusters, with two distinct clades within two clusters. The study indicated low genetic diversity, with expected and observed heterozygosity values ranging from 0.270 to 0.296 and 0.205 to 0.299, respectively. The unbiased gene diversity was 0.290. Principal component and ADMIXTURE analysis suggested that the 192 regional accessions could be categorized into four distinct divisions among accessions when K = 4, with low differentiation, as indicated by fixation index (F<sub>ST</sub>) values ranging from 0.011 to 0.131. The study found no significant genetic differences among cultivated enset regional accessions in southern Ethiopia. Enset genotyping using tGBS criteria with a specific enzyme was effective in yielding multiple sets of SNPs for enset landraces. The identified reliable SNPs could be beneficial for future enset breeding projects in Ethiopia.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Molecular characterization of cultivated Enset (Ensete ventricosum (Welw.) Cheesman) landraces through tunable genotyping-by-sequencing (tGBS) technology using single-nucleotide polymorphism (SNP) markers in Southern Ethiopia

  • 期刊论文

  • 发表日期：

    2026年1月7日

查看全文
  • Sisay Buta,
  • Zerihun Yemataw,
  • Andargachew Gedebo

摘要

Enset (Ensete ventricosum) is a crucial crop in Ethiopia, providing food security for rural communities. Despite its significance for nutrition and income, enset is often undervalued and underutilized. The research aimed to evaluate the genetic diversity, population structure, and relationships of these enset landraces. A recent study focused on genotyping 192 autochthonous enset accessions collected from various regions in Southern Ethiopia. The study evaluated the effectiveness of Single-nucleotide polymorphisms (SNPs) markers using tGBS (tunable genotyping by-sequencing) genotyping technology with the Bsp1286I restriction enzyme. Libraries were sequenced, quality-trimmed, and aligned to the Ensete ventricosum reference genome. SNP calling identified 129,649 high-quality markers from 788,103,162 reads generated from tGBS-sequences. These markers were used for the diversity analysis. relationships among regional accessions. The analysis of molecular variance (AMOVA) revealed minimal genetic differentiation among regional accessions 0.6%, with 99.4% of the genetic variation within accessions. Phylogenetic analysis grouped the regional enset accessions into three clusters, with two distinct clades within two clusters. The study indicated low genetic diversity, with expected and observed heterozygosity values ranging from 0.270 to 0.296 and 0.205 to 0.299, respectively. The unbiased gene diversity was 0.290. Principal component and ADMIXTURE analysis suggested that the 192 regional accessions could be categorized into four distinct divisions among accessions when K = 4, with low differentiation, as indicated by fixation index (FST) values ranging from 0.011 to 0.131. The study found no significant genetic differences among cultivated enset regional accessions in southern Ethiopia. Enset genotyping using tGBS criteria with a specific enzyme was effective in yielding multiple sets of SNPs for enset landraces. The identified reliable SNPs could be beneficial for future enset breeding projects in Ethiopia.