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<p>In Amapá, the cassava active germplasm bank (BAG) for direct consumption has been characterized using phenological markers; however, knowledge of its molecular characterization to identify genetic diversity and possible similarities is imperative. The objectives of this study were to: (i) identify if there is genetic variability in the BAG; (ii) identify by groupings whether there is similarity between clones and subsequently, based on morphological evaluations, to retain those with the greatest genetic differentiation to compose the BAG; (iii) identify the differences between the possible groups and which ones are furthest from the whole cluster. The BAG is in Mazagão municipality, Amapá State, Brazil, containing 62 genotypes with 10 plants for each one, where 20–40 leaf discs from fully opened young fresh healthy leaves were collected and sent for molecular analysis using the Diversity Arrays Technology (DArT) technique. Findings revealed four main groups which indicates that the BAG truly contains variability; there are pairs with high genetic similarity, which must be morphologically evaluated to decide whether to maintain or exclude BAG and; about the cluster, group 4 is in a central position of the graphical analysis surrounded by groups 1 and 3; because it is distant from the other groups the group 2 is less similar, and had greater heterozygosity, without inbreeding and genetic drift and erosion. The groups 1 and 4 have a greater number of alleles and private alleles, with genetic variation, inbreeding present, thus the number of individuals is responsible by variability.</p>

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Genetic diversity and population structure research in active bank germplasm (BAG) cassava genotypes using diversity arrays technology (DArT)

  • 期刊论文

  • 发表日期：

    2026年1月5日

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  • Gilberto Ken Iti Yokomizo,
  • Ana Flávia Francisconi,
  • Daniela Loschtschagina Gonzaga,
  • Maria Imaculada Zucchi,
  • José Baldin Pinheiro,
  • Eder Jorge de Oliveira

摘要

In Amapá, the cassava active germplasm bank (BAG) for direct consumption has been characterized using phenological markers; however, knowledge of its molecular characterization to identify genetic diversity and possible similarities is imperative. The objectives of this study were to: (i) identify if there is genetic variability in the BAG; (ii) identify by groupings whether there is similarity between clones and subsequently, based on morphological evaluations, to retain those with the greatest genetic differentiation to compose the BAG; (iii) identify the differences between the possible groups and which ones are furthest from the whole cluster. The BAG is in Mazagão municipality, Amapá State, Brazil, containing 62 genotypes with 10 plants for each one, where 20–40 leaf discs from fully opened young fresh healthy leaves were collected and sent for molecular analysis using the Diversity Arrays Technology (DArT) technique. Findings revealed four main groups which indicates that the BAG truly contains variability; there are pairs with high genetic similarity, which must be morphologically evaluated to decide whether to maintain or exclude BAG and; about the cluster, group 4 is in a central position of the graphical analysis surrounded by groups 1 and 3; because it is distant from the other groups the group 2 is less similar, and had greater heterozygosity, without inbreeding and genetic drift and erosion. The groups 1 and 4 have a greater number of alleles and private alleles, with genetic variation, inbreeding present, thus the number of individuals is responsible by variability.