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<p>Fusarium wilt, caused by <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> (Foc), remains a major constraint to global banana (<i>Musa spp</i>.) production, with Tropical Race 4 (TR4) posing the greatest threat to commercial cultivars. Developing resistant cultivars requires reliable molecular markers to distinguish susceptible from resistant genotypes. In this study, seven <i>Musa </i>leucine-rich repeat receptor-like protein (MaLRR-RLP) markers, derived from a resistance-associated locus on chromosome 10, were evaluated across 42 banana accessions representing diverse susceptible and resistant of wild and cultivated genotypes. Four markers (Ma10_p00450.1, Ma10_p00470.1, Ma10_p00490.1, Ma10_p00570.1) were monomorphic, while three (Ma10_p00500.1, Ma10_p00550.1, and Ma10_p00480.1) displayed polymorphism. Among these, Ma10_p00480.1 showed the strongest association with putative<i> Fusarium </i>wilt resistance, producing two distinct alleles: an ~ 500&#xa0;bp fragment unique to susceptible Cavendish cultivars, likely reflecting a deletion linked to susceptibility, and an ~ 600&#xa0;bp fragment found in <i>M. acuminata</i> subsp. <i>malaccensis</i>, potentially linked to resistance. Cluster analysis using Gower’s dissimilarity further separated resistant wild accessions from susceptible cultivated types, underscoring the functional relevance of this locus. These results identify Ma10_p00480.1 as a promising marker for marker-assisted selection and genetic resource characterization, supporting both the development of Foc-resistant cultivars and the conservation of valuable wild Musa germplasm.</p>

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Development of PCR-based putative molecular markers linked to fusarium wilt resistance in banana

  • 期刊论文

  • 发表日期：

    2025年12月9日

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  • Tri Handayani,
  • Diyah Martanti,
  • Herlina Herlina,
  • Fajarudin Ahmad,
  • Yuyu Suryasari Poerba,
  • Awang Maharijaya,
  • Yudiwanti Wahyu,
  • Sobir Sobir,
  • Witjaksono Witjaksono

摘要

Fusarium wilt, caused by Fusarium oxysporum f. sp. cubense (Foc), remains a major constraint to global banana (Musa spp.) production, with Tropical Race 4 (TR4) posing the greatest threat to commercial cultivars. Developing resistant cultivars requires reliable molecular markers to distinguish susceptible from resistant genotypes. In this study, seven Musa leucine-rich repeat receptor-like protein (MaLRR-RLP) markers, derived from a resistance-associated locus on chromosome 10, were evaluated across 42 banana accessions representing diverse susceptible and resistant of wild and cultivated genotypes. Four markers (Ma10_p00450.1, Ma10_p00470.1, Ma10_p00490.1, Ma10_p00570.1) were monomorphic, while three (Ma10_p00500.1, Ma10_p00550.1, and Ma10_p00480.1) displayed polymorphism. Among these, Ma10_p00480.1 showed the strongest association with putative Fusarium wilt resistance, producing two distinct alleles: an ~ 500 bp fragment unique to susceptible Cavendish cultivars, likely reflecting a deletion linked to susceptibility, and an ~ 600 bp fragment found in M. acuminata subsp. malaccensis, potentially linked to resistance. Cluster analysis using Gower’s dissimilarity further separated resistant wild accessions from susceptible cultivated types, underscoring the functional relevance of this locus. These results identify Ma10_p00480.1 as a promising marker for marker-assisted selection and genetic resource characterization, supporting both the development of Foc-resistant cultivars and the conservation of valuable wild Musa germplasm.