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<p><i>Typhonium flagelliforme,</i> a medicinal plant endemic to Indonesia and belonging to the Araceae family, has garnered significant attention due to its potential anticancer properties. Given its therapeutic relevance, this species represents a promising genetic resource for future plant breeding initiatives. In the present study, whole genome sequencing (WGS) of <i>T. flagelliforme</i> was performed using the Illumina NextSeq 2000 platform. Sequencing was conducted with a paired-end 150&#xa0;bp (PE150) approach, yielding approximately 112&#xa0;GB of raw data. The estimated genome size was 714.70&#xa0;Mb, with an assembly contig N50 of 3,971&#xa0;bp and a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness score of 76.08%. Also, we identified 64.41% repetitive DNA from the genome assembly, in which retroelements occupied 21.40% of the total genome. A total of 97,631 genomic simple sequence repeats (gSSRs) were identified. This first <i>T. flagelliforme</i> genome is expected to contribute to a better understanding of its genetics for molecular breeding programs, development of medicinal plant-based biotechnology, and sustainable conservation of rodent tubber germplasm.</p>

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First short-read genome assembly and simple sequence repeat (SSR) mining of Typhonium flagelliforme, an anti-cancer medicinal plant

  • 期刊论文

  • 发表日期：

    2025年12月15日

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  • Devit Purwoko,
  • Siti Zulaeha,
  • Gemilang Rahmadara,
  • Suparjo,
  • Teuku Tajuddin,
  • Syahnada Jaya Syaifullah,
  • Ani Kurniawati,
  • Willy Bayuardi Suwarno,
  • Sobir

摘要

Typhonium flagelliforme, a medicinal plant endemic to Indonesia and belonging to the Araceae family, has garnered significant attention due to its potential anticancer properties. Given its therapeutic relevance, this species represents a promising genetic resource for future plant breeding initiatives. In the present study, whole genome sequencing (WGS) of T. flagelliforme was performed using the Illumina NextSeq 2000 platform. Sequencing was conducted with a paired-end 150 bp (PE150) approach, yielding approximately 112 GB of raw data. The estimated genome size was 714.70 Mb, with an assembly contig N50 of 3,971 bp and a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness score of 76.08%. Also, we identified 64.41% repetitive DNA from the genome assembly, in which retroelements occupied 21.40% of the total genome. A total of 97,631 genomic simple sequence repeats (gSSRs) were identified. This first T. flagelliforme genome is expected to contribute to a better understanding of its genetics for molecular breeding programs, development of medicinal plant-based biotechnology, and sustainable conservation of rodent tubber germplasm.