<p>Iran possesses one of the richest genetic resources for pistachio trees and is a major producer of pistachio nuts worldwide. This study aimed to evaluate the genetic diversity and population structure of pistachio Saccessions from key provinces: East Azerbaijan, Semnan, Alborz, Qazvin, and Kerman, using start codon targeted (SCoT) and sequence-related amplified polymorphism (SRAP) markers. The primers used in this study successfully amplified 162 loci for SCoT and 107 loci for SRAP, yielding an average of 8.1 polymorphic fragments per primer for SCoT and 8.9 for SRAP. The polymorphic information content indicates that the markers utilized in this study are indeed polymorphic. The genetic diversity observed within individual populations greater than the variation found between populations. The results of genetic distance and clustering analyses corresponded with the geographical distribution of the accessions. Additionally, population structure analysis, conducted using the Bayesian algorithm, revealed a cluster structure: K = 4 for SCoT markers and K = 3 for SRAP markers, indicating that pistachio accessions from different locations were grouped together. These findings can assist in managing the genetic diversity of both wild and cultivated pistachios in the studied regions. Furthermore, the results suggest two locations as potential sources of genetic diversity that could be leveraged in breeding programs.</p>

错误:搜索内容不能为空,请输入英文关键词
错误:关键词超出字数限制,请精简
高级检索

Unraveling the genetic diversity and population structure of wild-type and cultivars of pistachio (Pistacia vera L.) using molecular markers

  • Mohammadreza Mansourian,
  • Reza Azizinezhad,
  • Mahmoud Khosroshaheli,
  • Asa Ebrahimi,
  • Eslam Majidi Hervan

摘要

Iran possesses one of the richest genetic resources for pistachio trees and is a major producer of pistachio nuts worldwide. This study aimed to evaluate the genetic diversity and population structure of pistachio Saccessions from key provinces: East Azerbaijan, Semnan, Alborz, Qazvin, and Kerman, using start codon targeted (SCoT) and sequence-related amplified polymorphism (SRAP) markers. The primers used in this study successfully amplified 162 loci for SCoT and 107 loci for SRAP, yielding an average of 8.1 polymorphic fragments per primer for SCoT and 8.9 for SRAP. The polymorphic information content indicates that the markers utilized in this study are indeed polymorphic. The genetic diversity observed within individual populations greater than the variation found between populations. The results of genetic distance and clustering analyses corresponded with the geographical distribution of the accessions. Additionally, population structure analysis, conducted using the Bayesian algorithm, revealed a cluster structure: K = 4 for SCoT markers and K = 3 for SRAP markers, indicating that pistachio accessions from different locations were grouped together. These findings can assist in managing the genetic diversity of both wild and cultivated pistachios in the studied regions. Furthermore, the results suggest two locations as potential sources of genetic diversity that could be leveraged in breeding programs.