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<p>Understanding the seed storage behaviour of <i>Euryale ferox</i> is essential for developing effective conservation strategies. This study was aimed at developing a standardized protocol for successful long-term conservation of <i>Euryale ferox</i> seeds. Freshly harvested seeds were assessed under three different storage temperatures viz ambient temperature (25–30&#xa0;°C), 4&#xa0;°C (medium-term storage module) and-18&#xa0;°C (long-term storage module)—combined with three seed moisture contents (on fresh weight basis): fresh (~ 27–35%), 10%, and 6%, over 20, 40, and 60&#xa0;days. Fresh seeds showed 98% germination and mean germination time (MGT) of 23&#xa0;days. Seeds stored at 6% moisture under − 18&#xa0;°C retained high viability (92–97% germination; MGT: 23–24&#xa0;days), statistically comparable to control seeds (<i>P</i> &gt; 0.05). Multivariate analysis of variance&#xa0;showed significant effects of seed moisture content (F = 62.22, <i>P</i> &lt; 0.001), storage condition (F = 56.63, <i>P</i> &lt; 0.001), and storage period (F = 11.53, <i>P</i> &lt; 0.001) on germination percentage. Histological research confirmed that desiccation to 6% moisture did not alter embryo or perisperm structure. A tetrazolium-based viability test was standardized using 0.1% triphenyl tetrazolium chloride (TTC) at 30&#xa0;°C for 2&#xa0;h following 24-h preconditioning, with results matching germination tests in seed lots with 0%, 24%, and 60% viability. Our study confirms that <i>Euryale ferox</i> seeds have orthodox seed storage behaviour and can be effectively conserved ex situ, in seed genebanks. The standardized viability testing and moisture determination protocols will facilitate efficient seed processing and long-term conservation of this economically important aquatic crop.</p>

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Elucidating the seed storage behaviour of Euryale ferox: implications for seed genebanking

  • 期刊论文

  • 发表日期：

    2025年8月29日

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  • Badal Singh,
  • S. Raj Kumar,
  • Mohd Harun,
  • Aradhana Mishra,
  • Veena Gupta,
  • Anjali Kak Koul,
  • Sherry Rachel Jacob,
  • Gyanendra Pratap Singh

摘要

Understanding the seed storage behaviour of Euryale ferox is essential for developing effective conservation strategies. This study was aimed at developing a standardized protocol for successful long-term conservation of Euryale ferox seeds. Freshly harvested seeds were assessed under three different storage temperatures viz ambient temperature (25–30 °C), 4 °C (medium-term storage module) and-18 °C (long-term storage module)—combined with three seed moisture contents (on fresh weight basis): fresh (~ 27–35%), 10%, and 6%, over 20, 40, and 60 days. Fresh seeds showed 98% germination and mean germination time (MGT) of 23 days. Seeds stored at 6% moisture under − 18 °C retained high viability (92–97% germination; MGT: 23–24 days), statistically comparable to control seeds (P > 0.05). Multivariate analysis of variance showed significant effects of seed moisture content (F = 62.22, P < 0.001), storage condition (F = 56.63, P < 0.001), and storage period (F = 11.53, P < 0.001) on germination percentage. Histological research confirmed that desiccation to 6% moisture did not alter embryo or perisperm structure. A tetrazolium-based viability test was standardized using 0.1% triphenyl tetrazolium chloride (TTC) at 30 °C for 2 h following 24-h preconditioning, with results matching germination tests in seed lots with 0%, 24%, and 60% viability. Our study confirms that Euryale ferox seeds have orthodox seed storage behaviour and can be effectively conserved ex situ, in seed genebanks. The standardized viability testing and moisture determination protocols will facilitate efficient seed processing and long-term conservation of this economically important aquatic crop.