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<p><i>Mentha spicata</i> L. (spearmint) is a commercially significant medicinal and aromatic plant, primarily valued for its high carvone content, a monoterpenoid ketone widely employed in the flavor, fragrance, and pharmaceutical industries. Precise identification of <i>M. spicata</i> is vital for ensuring cultivar purity, maintaining genetic integrity in breeding programs, and preventing adulteration in commercial supply chains. However, taxonomic resolution within the genus <i>Mentha</i> is impeded by extensive interspecific hybridization, polyploidy, and morphological convergence. In the present study, a species-specific Sequence Characterized Amplified Region (SCAR) marker was developed for the unambiguous molecular identification of <i>M. spicata</i>. Fourteen Inter-Simple Sequence Repeat (ISSR) primers were screened across six <i>Mentha</i> species, generating 75 polymorphic loci with an average Polymorphic Information Content (PIC) of 0.47, reflecting moderate genetic diversity. Among them, ISSR primer 5 amplified a unique 750&#xa0;bp fragment specific to <i>M. spicata</i> accessions. This species-exclusive fragment was excised, cloned, sequenced, and used to design SCAR-specific primers. The resulting SCAR marker consistently amplified a single 279&#xa0;bp band exclusively in <i>M. spicata</i>, demonstrating high specificity and reproducibility across diverse genotypes. This is the first report of an ISSR-derived SCAR marker for <i>M. spicata</i>, offering a reliable molecular tool for species authentication, germplasm validation, and regulatory compliance. Furthermore, its application in marker-assisted selection will support the development of elite cultivars with elevated carvone content, contributing to both conservation and commercial breeding programs in this economically valuable species.</p> Graphical abstract <p></p>

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A novel species-specific SCAR marker-based unambiguous molecular identification and conservation of Mentha spicata L

  • 期刊论文

  • 发表日期：

    2025年8月31日

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  • Varshika Singh,
  • Yash Pandey,
  • Nilesh Sharma,
  • Nivedita Singh,
  • Anil Kumar Gupta,
  • Gunjan Tiwari

摘要

Mentha spicata L. (spearmint) is a commercially significant medicinal and aromatic plant, primarily valued for its high carvone content, a monoterpenoid ketone widely employed in the flavor, fragrance, and pharmaceutical industries. Precise identification of M. spicata is vital for ensuring cultivar purity, maintaining genetic integrity in breeding programs, and preventing adulteration in commercial supply chains. However, taxonomic resolution within the genus Mentha is impeded by extensive interspecific hybridization, polyploidy, and morphological convergence. In the present study, a species-specific Sequence Characterized Amplified Region (SCAR) marker was developed for the unambiguous molecular identification of M. spicata. Fourteen Inter-Simple Sequence Repeat (ISSR) primers were screened across six Mentha species, generating 75 polymorphic loci with an average Polymorphic Information Content (PIC) of 0.47, reflecting moderate genetic diversity. Among them, ISSR primer 5 amplified a unique 750 bp fragment specific to M. spicata accessions. This species-exclusive fragment was excised, cloned, sequenced, and used to design SCAR-specific primers. The resulting SCAR marker consistently amplified a single 279 bp band exclusively in M. spicata, demonstrating high specificity and reproducibility across diverse genotypes. This is the first report of an ISSR-derived SCAR marker for M. spicata, offering a reliable molecular tool for species authentication, germplasm validation, and regulatory compliance. Furthermore, its application in marker-assisted selection will support the development of elite cultivars with elevated carvone content, contributing to both conservation and commercial breeding programs in this economically valuable species.

Graphical abstract