Scholarlink

<p><i>Codonopsis pilosula</i> is a renowned traditional Chinese herbal medicine indigenous to the north of China. For the purpose of advancing crossbreeding efforts in this species, we investigated its low self-fertilization rate and its hypothesized mechanism of intrinsic self-incompatibility. Two Gansu cultivars were utilized in a field pollination experiment, wherein pollen viability, pollen germination, and stigma acceptability were assessed, and the flowering cycle was observed. The obtained results confirmed the occurrence of dichogamy and secondary pollen presentation in <i>C. pilosula</i>. Further, transcriptome sequencing analysis was performed to identify the putative <i>S</i> genes transcripts in their pistil and stamen tissues, with the observed presence of the RNase T2 and SLF indicating the likely existence of the S-RNase-based self-incompatibility system in this species. These provide instructive insight for future crossbreeding in this important cultivated medicinal plant.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Low self-fertilization rate and putative self-incompatibility in Codonopsis pilosula (Campanulaceae)

  • 期刊论文

  • 发表日期：

    2025年7月25日

查看全文
  • Hui Zhang,
  • Shaoming Shi,
  • Lujie Yang,
  • Fan Zhao,
  • Zhiqi Wang,
  • Ying Zhang,
  • Jing Fang,
  • Yujie Zhang,
  • Lingling Da

摘要

Codonopsis pilosula is a renowned traditional Chinese herbal medicine indigenous to the north of China. For the purpose of advancing crossbreeding efforts in this species, we investigated its low self-fertilization rate and its hypothesized mechanism of intrinsic self-incompatibility. Two Gansu cultivars were utilized in a field pollination experiment, wherein pollen viability, pollen germination, and stigma acceptability were assessed, and the flowering cycle was observed. The obtained results confirmed the occurrence of dichogamy and secondary pollen presentation in C. pilosula. Further, transcriptome sequencing analysis was performed to identify the putative S genes transcripts in their pistil and stamen tissues, with the observed presence of the RNase T2 and SLF indicating the likely existence of the S-RNase-based self-incompatibility system in this species. These provide instructive insight for future crossbreeding in this important cultivated medicinal plant.