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<p>DREBs constitute a category of transcription factors widespread known to exist in the Plantae and exert major roles in signal transduction, plant growth and developing, and in responding from stress. DREB-family genes have been identified and characterized in various genera. Nevertheless, genome-wide characterization for DREB genes as well as its expression profiles in distinct tissues in the important medicinal and food-type crop <i>Dioscorea alata</i> remains relatively limited, and few clones of this gene family have been reported. In light of this research, a thorough genome-wide analyses of the DREB family gene in <i>Dioscorea alata</i> L. identified 47 DaDREB genes and provided insights into gene structures, protein motifs, phylogenetic relationships, along with stress-related cis-regulatory elements of these genes. Taking advantage of bioinformatics analyzes, we researched the expression profiles of DaDREBs across various tissues with the aid of RNA-seq and qRT-PCR. The findings suggested that such genes might be engaged in the stress response, growing and developing of <i>D. alata</i>. In addition, the induced expressed of <i>DaDREB26</i> was obviously up-regulated under drought stress, and we cloned and biologically analyzed it. Results of our study provide a holistic and systematic picture of the evolutionary trajectory of DREB genes in <i>D</i>. <i>alata</i> and the prospective functions of the DREB family genes in stress and hormone responses. For the first time, we report the response of <i>DaDREB26</i> to drought stress in <i>D</i>. <i>alata</i>, which forms the basis for further exploiting the functions of DaDREBs under abiotic stress.</p>

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Genome-wide comprehensive analysis of DREB gene family in greater yam: exploring the role of DaDREB26 in plant drought stress response

  • 期刊论文

  • 发表日期：

    2025年6月19日

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  • Qiang Zhang,
  • Hongling Tian,
  • Shuhong Guo,
  • Yaoqin Wang,
  • Shuaishuai Pei,
  • Changjuan Wu,
  • Qiubao Wang,
  • Xianqiang Zuo

摘要

DREBs constitute a category of transcription factors widespread known to exist in the Plantae and exert major roles in signal transduction, plant growth and developing, and in responding from stress. DREB-family genes have been identified and characterized in various genera. Nevertheless, genome-wide characterization for DREB genes as well as its expression profiles in distinct tissues in the important medicinal and food-type crop Dioscorea alata remains relatively limited, and few clones of this gene family have been reported. In light of this research, a thorough genome-wide analyses of the DREB family gene in Dioscorea alata L. identified 47 DaDREB genes and provided insights into gene structures, protein motifs, phylogenetic relationships, along with stress-related cis-regulatory elements of these genes. Taking advantage of bioinformatics analyzes, we researched the expression profiles of DaDREBs across various tissues with the aid of RNA-seq and qRT-PCR. The findings suggested that such genes might be engaged in the stress response, growing and developing of D. alata. In addition, the induced expressed of DaDREB26 was obviously up-regulated under drought stress, and we cloned and biologically analyzed it. Results of our study provide a holistic and systematic picture of the evolutionary trajectory of DREB genes in D. alata and the prospective functions of the DREB family genes in stress and hormone responses. For the first time, we report the response of DaDREB26 to drought stress in D. alata, which forms the basis for further exploiting the functions of DaDREBs under abiotic stress.