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<p>Bananas and plantains, which belong to the <i>Musa</i> genus, serve as essential global food sources, valued for their nutritional benefits and culinary uses. However, they face threats from diseases such as black sigatoka and <i>Fusarium</i> wilt, particularly from the highly virulent <i>Fusarium oxysporum</i> f. sp. <i>cubense</i> tropical race 4 (FOC-TR4), which has been reported in several Central American regions and has emerged in Venezuela. The potential spread of this strain to Puerto Rico has prompted researchers to explore resistant cultivars. Unfortunately, the understanding of resistance gene characterization and detection in Puerto Rico is limited. Molecular techniques, including PCR, qPCR, Sanger sequencing, and phylogenetics, were utilized to study in-vitro <i>Musa</i> spp. (Dwarf, Maiden, and Maricongo). This study aimed to identify <i>resistance gene analog 2</i> (<i>RGA</i>2) and assess its abundance and evolution in <i>Musa</i> spp. from Puerto Rico. The results showed no significant variation in transcript levels of <i>RGA2</i>; however, phylogenetic analysis revealed that the Maricongo cultivar displayed considerable similarity to previously identified candidate resistance genes. This suggests that <i>RGA2</i> is present in local cultivars, indicating its association with R-genes related to <i>Fusarium</i> resistance. This research contributes to the advancement of our understanding of the genetic diversity of resistance genes within local <i>Musa</i> spp., thereby establishing a foundation for subsequent research endeavors. Additionally, it offers valuable tools and insights to address both present and future challenges faced in the Caribbean region.</p>

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Protecting Puerto Rico agriculture: A predictive perspective on resistance gene candidate RGA2 against Fusarium oxysporum f. sp. cubense tropical race 4 in Musa spp.

  • 期刊论文

  • 发表日期：

    2025年5月29日

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  • Christopher A. Sambolín Pérez,
  • Rosalinda Aybar Batista,
  • Juan A. Negrón Berríos

摘要

Bananas and plantains, which belong to the Musa genus, serve as essential global food sources, valued for their nutritional benefits and culinary uses. However, they face threats from diseases such as black sigatoka and Fusarium wilt, particularly from the highly virulent Fusarium oxysporum f. sp. cubense tropical race 4 (FOC-TR4), which has been reported in several Central American regions and has emerged in Venezuela. The potential spread of this strain to Puerto Rico has prompted researchers to explore resistant cultivars. Unfortunately, the understanding of resistance gene characterization and detection in Puerto Rico is limited. Molecular techniques, including PCR, qPCR, Sanger sequencing, and phylogenetics, were utilized to study in-vitro Musa spp. (Dwarf, Maiden, and Maricongo). This study aimed to identify resistance gene analog 2 (RGA2) and assess its abundance and evolution in Musa spp. from Puerto Rico. The results showed no significant variation in transcript levels of RGA2; however, phylogenetic analysis revealed that the Maricongo cultivar displayed considerable similarity to previously identified candidate resistance genes. This suggests that RGA2 is present in local cultivars, indicating its association with R-genes related to Fusarium resistance. This research contributes to the advancement of our understanding of the genetic diversity of resistance genes within local Musa spp., thereby establishing a foundation for subsequent research endeavors. Additionally, it offers valuable tools and insights to address both present and future challenges faced in the Caribbean region.