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<p>The aim of the present study was to evaluate the genetic diversity of genotypes of <i>Dioscorea rotundata</i> Poir. originating from states of the Brazilian Northeast region (Sergipe, Bahia, Alagoas, and Paraíba) through morphological and molecular characterization. For this evaluation, 52 yam genotypes were collected from market locations and directly from rural growers. Morphological characterization was performed using 31 descriptors specific to the yam crop. The environmental coefficient of variation (CV<sub>e</sub>) of most of the descriptors evaluated was greater than the genotypic coefficient of variation (CV<sub>g</sub>), and heritability was high only for stem color (86.86%). The highest significant positive correlation was between the presence of the tuber and the presence of roots on the surface of the tuber (r = 1; <i>p</i> &lt; 0.001). For molecular characterization, 9 simple sequence repeat (SSR) and 16 ISSR inter simple sequence repeat (primers)&#xa0;were used. The primers used were considered informative, showing high polymorphic information content; and 100% of the bands were polymorphic. Genetic diversity was estimated using the following parameters: Number of alleles (Na = 2.00), effective number of alleles (Ne = 1.30), expected heterozygosity (He = 0.211), and Shannon’s information index (I = 0.351). The 52 genotypes were clustered in seven groups according to the Jaccard distance. The population’s genetic structure in K = 2 reveals shared alleles among the genotypes of different geographic origins. Morphological and molecular characterization demonstrated high genetic diversity among the 52 <i>Dioscorea</i> sp. genotypes but low diversity among collection sites.</p>

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Genetic diversity of yam (Dioscorea rotundata Poir.) through morphological and molecular markers

  • 期刊论文

  • 发表日期：

    2025年5月8日

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  • Crislaine Alves dos Santos,
  • Itamara Bomfim Gois,
  • Laura Catharine Doria Prata,
  • Crislaine Costa Calazans,
  • Ana Veruska Cruz da Silva,
  • Renata Silva-Mann,
  • Arie Fitzgerald Blank,
  • Maria de Fátima Arrigoni-Blank

摘要

The aim of the present study was to evaluate the genetic diversity of genotypes of Dioscorea rotundata Poir. originating from states of the Brazilian Northeast region (Sergipe, Bahia, Alagoas, and Paraíba) through morphological and molecular characterization. For this evaluation, 52 yam genotypes were collected from market locations and directly from rural growers. Morphological characterization was performed using 31 descriptors specific to the yam crop. The environmental coefficient of variation (CVe) of most of the descriptors evaluated was greater than the genotypic coefficient of variation (CVg), and heritability was high only for stem color (86.86%). The highest significant positive correlation was between the presence of the tuber and the presence of roots on the surface of the tuber (r = 1; p < 0.001). For molecular characterization, 9 simple sequence repeat (SSR) and 16 ISSR inter simple sequence repeat (primers) were used. The primers used were considered informative, showing high polymorphic information content; and 100% of the bands were polymorphic. Genetic diversity was estimated using the following parameters: Number of alleles (Na = 2.00), effective number of alleles (Ne = 1.30), expected heterozygosity (He = 0.211), and Shannon’s information index (I = 0.351). The 52 genotypes were clustered in seven groups according to the Jaccard distance. The population’s genetic structure in K = 2 reveals shared alleles among the genotypes of different geographic origins. Morphological and molecular characterization demonstrated high genetic diversity among the 52 Dioscorea sp. genotypes but low diversity among collection sites.