<p>The&#xa0;present study was undertaken to develop cryopreservation protocol of <i>Artocarpus lacucha</i> Buch. –Ham. using three vitrification-based cryopreservation techniques viz., Vitrification, Droplet Vitrification and V- cryoplate. The most efficient protocol was optimised using V- cryoplate technique. Shoot tip were isolated from non-cold-acclimated and 1, 2 and 3-weeks-cold-acclimated cultures. Shoot tips were precultured on 0.3&#xa0;M sucrose for overnight. Dehydration was performed by immersing cryo-plates in loading solution for 20 min followed by plant vitrification solution-2 (PVS2) treatment for 20–60 min followed by rapid freezing and thawing. Significant effect of cold acclimation and PVS2 duration was observed on survival and regrowth after cryopreservation. Maximum post-thaw regeneration (35.2%) was observed in shoot tips of 1-week-cold-acclimated cultures dehydrated with chilled PVS2 for 30 min. PVS2 toxicity was observed in longer-duration treatments. The results of the present study exhibited that cryopreservation protocol employing aluminium cryoplates can effectively be applied for long-term conservation of <i>A. lacucha</i> germplasm.</p>