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<p>Lettuce (<i>Lactuca sativa</i> var. <i>ramosa</i> Hort) is an important and popular leafy vegetable with highly nutritional value and economic value. In this study, the whole chloroplast (cp) genome of <i>L. sativa</i> var. <i>ramosa</i> was sequenced and assembled. The <i>L. sativa</i> var. <i>ramosa</i> cp genome was 152,765&#xa0;bp in length, containing a large single-copy region (LSC, 84,103&#xa0;bp), a small single-copy region (SSC, 18,596&#xa0;bp), and two inverted repeat regions (IRa and IRb, both are 25,033&#xa0;bp). Our annotation indicated that the <i>L. sativa</i> var. <i>ramosa</i> cp genome encoded 133 genes, consisting of 87 protein-coding genes (PCGs), 37 tRNA genes, 8 rRNA genes and 1 pseudo gene. A total of 45 dispersed repeats and 191 simple sequence repeats&#xa0;(SSRs) were identified. In addition, our analyses revealed that the most abundant type of SSRs was A/T mononucleotides. Five highly variable regions, namely, (<i>rps12</i>, <i>ycf3</i>, <i>petG</i>, <i>ycf1</i>, and <i>ycf15</i>) were selected as potential cp markers. The IR boundaries between <i>L. sativa</i> var. <i>ramosa</i> and six other <i>Lactuca</i> species were systematically analyzed. Based on the result of phylogenetic analysis, <i>L. sativa</i> var. <i>ramosa</i> was most closely related to <i>L. sativa.</i> cv Salinas. Our findings provide the complete cp genome of <i>L. sativa</i> var. <i>ramosa,</i> offering resources for developing molecular markers and understanding evolutionary relationships.</p>

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Complete chloroplast genome sequence and phylogenetic analysis of Lactuca sativa var. ramosa hort

  • 期刊论文

  • 发表日期：

    2025年3月30日

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  • Yihui Gong,
  • Yalin Qin,
  • Tao Luo,
  • Peng Li,
  • Guihua Zhou

摘要

Lettuce (Lactuca sativa var. ramosa Hort) is an important and popular leafy vegetable with highly nutritional value and economic value. In this study, the whole chloroplast (cp) genome of L. sativa var. ramosa was sequenced and assembled. The L. sativa var. ramosa cp genome was 152,765 bp in length, containing a large single-copy region (LSC, 84,103 bp), a small single-copy region (SSC, 18,596 bp), and two inverted repeat regions (IRa and IRb, both are 25,033 bp). Our annotation indicated that the L. sativa var. ramosa cp genome encoded 133 genes, consisting of 87 protein-coding genes (PCGs), 37 tRNA genes, 8 rRNA genes and 1 pseudo gene. A total of 45 dispersed repeats and 191 simple sequence repeats (SSRs) were identified. In addition, our analyses revealed that the most abundant type of SSRs was A/T mononucleotides. Five highly variable regions, namely, (rps12, ycf3, petG, ycf1, and ycf15) were selected as potential cp markers. The IR boundaries between L. sativa var. ramosa and six other Lactuca species were systematically analyzed. Based on the result of phylogenetic analysis, L. sativa var. ramosa was most closely related to L. sativa. cv Salinas. Our findings provide the complete cp genome of L. sativa var. ramosa, offering resources for developing molecular markers and understanding evolutionary relationships.