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<p>The <i>HVA22</i> gene family, initially identified in barley, plays a crucial role in plant stress response mechanisms, aiding plants in withstanding various environmental pressures. However, this gene family has not been studied in <i>Brassica napus</i> until now. In this study, we identified 39 <i>HVA22</i> genes (<i>BnHVA22.1</i>–<i>BnHVA22.39</i>) distributed across the <i>B. napus</i> genome. Phylogenetic analysis categorized these genes into four distinct clades, which include both monocotyledon and dicotyledon species. The <i>BnHVA22</i> genes are characterized by a variable number of introns, ranging from three to seven, with intron phases predominantly occurring in phases 1, 0, and 2. Furthermore, the proteins encoded by these genes possess a conserved TB2/DP1/HVA22 domain. The expansion of the <i>BnHVA22</i> gene family is primarily attributed to segmental/whole-genome duplications. Duplicated orthologous gene pairs exhibit negative selection, with an average constraint of 0.26, indicating functional conservation of these genes throughout evolution. Stress- and abscisic acid (ABA)-responsive <i>cis</i>-regulatory elements are key regulators of <i>BnHVA22</i> gene transcription, while microRNAs such as <i>miRNA167</i> modulate gene expression at the post-transcriptional level. Transcriptomic analysis has demonstrated the involvement of <i>BnHVA22</i> genes in <i>B. napus</i> growth, development, and responses to abiotic stress. Moreover, qRT-PCR analysis confirmed the significant differential expression of <i>BnHVA22.8</i>, <i>BnHVA22.14</i>, <i>BnHVA22.15</i>, <i>BnHVA22.30</i>, and <i>BnHVA22.39</i> in response to ABA and salt stress. These findings elucidate the pivotal role of the <i>HVA22</i> gene family in the stress tolerance of <i>B. napus</i>, thus&#xa0;providing valuable insights for breeding stress-resistant <i>B. napus</i> cultivars.</p>

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Genome-wide identification, evolution, and expression analysis of HVA22 gene family in Brassica napus L.

  • 期刊论文

  • 发表日期：

    2025年3月25日

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  • Ping Wang,
  • Lirong Wang

摘要

The HVA22 gene family, initially identified in barley, plays a crucial role in plant stress response mechanisms, aiding plants in withstanding various environmental pressures. However, this gene family has not been studied in Brassica napus until now. In this study, we identified 39 HVA22 genes (BnHVA22.1–BnHVA22.39) distributed across the B. napus genome. Phylogenetic analysis categorized these genes into four distinct clades, which include both monocotyledon and dicotyledon species. The BnHVA22 genes are characterized by a variable number of introns, ranging from three to seven, with intron phases predominantly occurring in phases 1, 0, and 2. Furthermore, the proteins encoded by these genes possess a conserved TB2/DP1/HVA22 domain. The expansion of the BnHVA22 gene family is primarily attributed to segmental/whole-genome duplications. Duplicated orthologous gene pairs exhibit negative selection, with an average constraint of 0.26, indicating functional conservation of these genes throughout evolution. Stress- and abscisic acid (ABA)-responsive cis-regulatory elements are key regulators of BnHVA22 gene transcription, while microRNAs such as miRNA167 modulate gene expression at the post-transcriptional level. Transcriptomic analysis has demonstrated the involvement of BnHVA22 genes in B. napus growth, development, and responses to abiotic stress. Moreover, qRT-PCR analysis confirmed the significant differential expression of BnHVA22.8, BnHVA22.14, BnHVA22.15, BnHVA22.30, and BnHVA22.39 in response to ABA and salt stress. These findings elucidate the pivotal role of the HVA22 gene family in the stress tolerance of B. napus, thus providing valuable insights for breeding stress-resistant B. napus cultivars.