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<p>Cytoplasmic male sterility (CMS) is used in breeding to facilitate hybrid seed production. <i>Restorer-of-fertility</i> (<i>Rf</i>), the suppressor of CMS, consists of a gene cluster with multiple haplotypes. Selection of restoring or non-restoring alleles depends on the discrimination of <i>Rf</i> haplotypes using DNA markers. The efficacy of this system is decreased if the <i>Rf</i> haplotype differs within the population of interest, which can occur during the crop evolution. In sugar beet, the <i>Rf1</i> gene has multiple haplotypes that are grouped by a linked polymorphic region (s17), with one group, termed p4, uniquely linked to the recessive <i>rf1</i> haplotype. Garden beet is the predecessor cultivar group of the sugar beet group. We questioned whether <i>Rf1</i> haplotypes differ between these two cultivar groups to assess the utility of marker-assisted selection. We analyzed 48 garden beet landraces and observed differences in the s17 polymorphism compared to sugar beet, suggesting that the <i>Rf1</i> haplotype frequency has changed during evolution of the crop. We next selected non-restoring genotypes from the garden beet landraces through test crosses and identified three recessive <i>rf1</i> haplotypes: one is identical to the p4 haplotype and the others are novel haplotypes. The p4 haplotype occurs in a few accessions and its frequency in garden beet is approximately 0.01. We analyzed the s17 polymorphism in modern garden beet hybrids and their constituents. We identified the p4 haplotype and suggest the presence of other recessive <i>rf1</i> haplotypes. Selection of p4 haplotype was efficient on the identification of non-restoring genotype in garden beet.</p>

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Differences in restorer-of-fertility 1 haplotype polymorphism are associated with crop history of garden beet and sugar beet (Beta vulgaris L.)

  • 期刊论文

  • 发表日期：

    2025年2月8日

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  • Eigo Taniguchi,
  • Yohei Kanomata,
  • Haruto Tanaka,
  • Mion Oishi,
  • Ryo Hayakawa,
  • Hiroaki Matsuhira,
  • Tsubasa Narihiro,
  • Yosuke Kuroda,
  • Hiroyo Kagami-Katsuyama,
  • Tomohiko Kubo,
  • Kazuyoshi Kitazaki

摘要

Cytoplasmic male sterility (CMS) is used in breeding to facilitate hybrid seed production. Restorer-of-fertility (Rf), the suppressor of CMS, consists of a gene cluster with multiple haplotypes. Selection of restoring or non-restoring alleles depends on the discrimination of Rf haplotypes using DNA markers. The efficacy of this system is decreased if the Rf haplotype differs within the population of interest, which can occur during the crop evolution. In sugar beet, the Rf1 gene has multiple haplotypes that are grouped by a linked polymorphic region (s17), with one group, termed p4, uniquely linked to the recessive rf1 haplotype. Garden beet is the predecessor cultivar group of the sugar beet group. We questioned whether Rf1 haplotypes differ between these two cultivar groups to assess the utility of marker-assisted selection. We analyzed 48 garden beet landraces and observed differences in the s17 polymorphism compared to sugar beet, suggesting that the Rf1 haplotype frequency has changed during evolution of the crop. We next selected non-restoring genotypes from the garden beet landraces through test crosses and identified three recessive rf1 haplotypes: one is identical to the p4 haplotype and the others are novel haplotypes. The p4 haplotype occurs in a few accessions and its frequency in garden beet is approximately 0.01. We analyzed the s17 polymorphism in modern garden beet hybrids and their constituents. We identified the p4 haplotype and suggest the presence of other recessive rf1 haplotypes. Selection of p4 haplotype was efficient on the identification of non-restoring genotype in garden beet.