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<p>Seven diploid accessions and their autotetraploid lines of Tartary buckwheat were used to conduct a karyotype study using the conventional squash method, establishing a reference for polyploid and wide hybridization breeding. In the present study, the seven cultivars exhibited similar karyotypic features in both diploid and autotetraploid accessions. The diploid chromosome number was 2n = 2x = 16, whereas it was 2n = 2x = 32 in the autotetraploid accessions. Diploids (T6, T9, T18, T22, T27 and T36) contained one pair of SAT chromosomes, whereas T25 diploid line had two pairs. Compared with diploids, the total number of chromosomes and SAT chromosomes doubled in autotetraploid. However, the karyotype asymmetry coefficient for the seven diploids ranged from 59.38 to 61.78%, and for the autotetraploid, it ranged from 59.73 to 61.76%. Additionally, the karyotype of autotetraploid remained unchanged in 2A type cases. Chromosome doubling did not affect the evolutionary relationship or clustering of Tartary buckwheat (<i>P</i> &lt; 0.01). The karyotypes and chromosome numbers of the seven autotetraploid lines have been reported for the first time.</p>

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Karyotype and evolution trend analysis of seven diploid accessions and their autotetraploid lines of Tartary buckwheat native to China

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  • 发表日期：

    2025年2月25日

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  • Li-kun Lian,
  • Pei Zhang,
  • Xiao-fen Wu,
  • Yi-zhong Zhang,
  • Qing-fu Chen

摘要

Seven diploid accessions and their autotetraploid lines of Tartary buckwheat were used to conduct a karyotype study using the conventional squash method, establishing a reference for polyploid and wide hybridization breeding. In the present study, the seven cultivars exhibited similar karyotypic features in both diploid and autotetraploid accessions. The diploid chromosome number was 2n = 2x = 16, whereas it was 2n = 2x = 32 in the autotetraploid accessions. Diploids (T6, T9, T18, T22, T27 and T36) contained one pair of SAT chromosomes, whereas T25 diploid line had two pairs. Compared with diploids, the total number of chromosomes and SAT chromosomes doubled in autotetraploid. However, the karyotype asymmetry coefficient for the seven diploids ranged from 59.38 to 61.78%, and for the autotetraploid, it ranged from 59.73 to 61.76%. Additionally, the karyotype of autotetraploid remained unchanged in 2A type cases. Chromosome doubling did not affect the evolutionary relationship or clustering of Tartary buckwheat (P < 0.01). The karyotypes and chromosome numbers of the seven autotetraploid lines have been reported for the first time.