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GRAS-Di analysis on recombinant inbred lines derived from the cross between Asian cultivated and wild rice species suitable for agronomic evaluation in high-latitude areas

  • Yohei Koide,
  • Yoshiki Tokuyama,
  • Miku Omachi,
  • Shiori Kushida,
  • Shuhei Okada,
  • Minami Taguchi,
  • Zin Mar Myint,
  • Thet Hter San,
  • Yuji Kishima,
  • Kazumitsu Onishi

摘要

Asian wild rice (Oryza rufipogon), a wild progenitor of Asian cultivated rice (O. sativa), is regarded as a unique genetic reservoir for future rice breeding. However, strong photoperiod sensitivity in O. rufipogon often inhibits genetic experiments, especially in high-latitude areas. Here, we found that the growth period of W630, a strain of O. rufipogon, is relatively short enough to conduct experiments in the high-latitude areas, such as northern limits of rice cultivation. Using the GRAS-Di, an NGS-based genotyping method, we determined the genome-wide genotypes of recombinant inbred lines derived from the cross between W630 and O. sativa. We identified qualitative trait loci for the agronomic traits possessed by W630. These results demonstrate the potential of early-growth wild rice for genetic research of agronomic traits to improve rice cultivation in high-latitude areas.