Citrus Greek National Germplasm Collection: a genetic diversity survey using nuclear and chloroplast microsatellite markers
摘要
In the present study, a combination of 17 nuclear and four chloroplast microsatellites were used to identify polymorphisms among 224 Citrus accessions maintained at the ex-situ ELGO-DIMITRA germplasm collection, mainly located in Chania, Greece. The nuclear marker data set revealed 109 unique genetic profiles with a mean gene diversity of 0.63, while the chloroplast DNA data set revealed 28 haplotypes between all accessions. A Bayesian genetic structure analysis was used to assign accessions into groups, and a dendrogram based on chloroplast markers allowed the maternal lineage identification of multiple hybrids. A few indigenous accessions exhibited notable genetic differences compared to the evaluated international counterparts (citron: ‘Cretan Smooth’, mandarin: ‘Common Chios’, lemon: ‘Karystini Xylokastrou’/‘Vakalou’/‘Helen’, lime: ‘Pastolemono Chiou’/‘Glykolemono’, greek bergamots, sweet orange: ‘Botsato Artas’). The rest of them presented highly similar or indistinguishable genetic profiles with known accessions. These results are expected to facilitate further exploration of Citrus genotypes maintained in Greece and assist in efficient conservation and design of breeding programs.